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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
在-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Rhesus ACE2 / Angiotensin-Converting Enzyme 2 Protein (Fc Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
20 µg/100 µg/1 mg
| 规格: | 20 µg | 产品价格: | ¥2830 |
|---|---|---|---|
| 规格: | 100 µg | 产品价格: | ¥7250 |
| 规格: | 1 mg | 产品价格: | ¥31590 |
蛋白名称:ACE2蛋白, ACE2 protein
蛋白构建:A DNA sequence encoding the rhesus ACE2 (ACI04564.1) (Met1-Val739) was expressed with the Fc region of human IgG1 at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 95 % as determined by SDS-PAGE
蛋白活性:Measured by its ability to cleave a fluorogenic peptide substrate, Mca-YVADAPK (Dnp)-OH (Catalog # ES007). The specific activity is >250 pmol/min/µg. Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2 (2019-nCoV) Spike Protein (S1+S2 ECD, His tag)(40589-V08B1) at 2μg/mL (100μL/well) can bind Rhesus ACE2-Fc,the EC50 of ACE2 is 30-80 ng/mL.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Gln 18
蛋白分子量:The recombinant Rhesus ACE2 is comprises 963 amino acids and has a calculated molecular mass of 110.7kDa.
蛋白NP号:ACI04564.1
蛋白氨基酸序列:Met1-Val739
蛋白标签:C-human IgG1-Fc
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验Identifying Protein Interactions by Hydroxyl‐Radical Protein Footprinting
Basic Protocol 1: Generation of an Active Recombinant Protein That is End‐Labeled Basic Protocol 2: Hydroxyl Radical Cleavage of Protein in Absence and Presence of Ligand Basic Protocol 3: Analyze Gel
Strategies to Optimize Protein Expression in E. coli
. Conformational stability of pGEX‐expressed Schistosoma japonicum glutathione S‐transferase: A detoxification enzyme and fusion‐protein affinity tag. Protein Sci. 6:399‐406. Kapust, R.B
Site‐Specific Protein Labeling with SNAP‐Tags
and specificities and provide flexibility not available with fluorescent proteins. The SNAP?tag is a modified form of the DNA repair enzyme human O 6 ?alkylguanine?DNA?alkyltransferase, and undergoes a self?labeling reaction to form a covalent bond with O
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