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- 2026年08月13日
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- 文献和实验
- 技术资料
- 保质期：
Upon receipt, store it immediately at -70°C or lower for long term storage. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: <br>2-8°C for 2-7 days; <br>-20°C for 1 months and -70℃ for 3 months.
- 保存条件：
Upon receipt, store it immediately at -70°C or lower for long term storage. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: <br>2-8°C for 2-7 days; <br>-20°C for 1 months and -70℃ for 3 months.
- 英文名：
Serpin A12
- 库存：
999
- 供应商：
北京索莱宝科技有限公司
- 规格：
10ug/50ug/500ug/1mg
| 规格： | 10ug | 产品价格： | ¥1217 |
|---|---|---|---|
| 规格： | 50ug | 产品价格： | ¥3401 |
| 规格： | 500ug | 产品价格： | ¥11933 |
| 规格： | 1mg | 产品价格： | ¥17021 |
| 基因名 | Serpin A12 |
| 别名 | Serpin A12; OL-64; Visceral Adipose Tissue-Derived Serine Protease Inhibitor; Vaspin; Visceral Adipose-Specific Serpin; SERPINA12 |
| 蛋白号 | Q8IW75 |
| 种属 | Human |
| 宿主 | E.coli |
| 产品描述 | Recombinant Human Serine Protease Inhibitor-clade A12 is produced by our E.coli expression system and the target gene encoding Leu21-Lys414 is expressed with a GST tag at the N-terminus. |
| 区间范围 | Leu21-Lys414 |
| 标签信息 | N-GST |
| 预测分子量 | 72 kDa |
| 表观分子量 | 72 KDa, reducing conditions |
| 纯度 | ≥80% by SDS-PAGE. |
| 内毒素 | <1EU/ug as determined by LAL test. |
| 溶剂 | Supplied as a 0.2 μm filtered solution of 50mM Tris-HCl, 160mM NaCl, 0.2mM PMSF, 1mM DTT, 10% Glycerine, pH 7.2. |
| 复溶 | Reconstituted protein solution can be diluted with distilled water. Always centrifuge tubes before opening. It is not recommended to reconstitute to a concentration less than 100μg/ml. |
| 储存条件 | Upon receipt, store it immediately at -70°C or lower for long term storage. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: 2-8°C for 2-7 days; -20°C for 1 months and -70℃ for 3 months. |
| 运输条件 | 干冰运输（需要收取干冰费） |
| 背景说明 | Vaspin (Visceral Adipose-Specific SERPIN) is a newly described adipokine. Vaspin has three β-sheets, nine α-helices, and one central loop; the structure is part of the set of distinctive features that are descriptive of Serpin family members. Vaspin is also a unique insulin sensitizing adipocytokine in obesity. A recent publication indicates that Vaspin mRNA expression in visceral fat is positively correlated with BMI and percent of body fat. and could be associated with parameters of obesity, insulin resistance, and glucose metabolism. These findings suggest a potential clinical use for Vaspin in ameliorating certain aberrations seen in the obesity metabolic syndrome. |
| 细胞活性 | No activity experimental data, to be verified. |
| ELISA活性 | No activity experimental data, to be verified. |
| SPR/BLI活性 | No activity experimental data, to be verified. |
| 研究领域 | 生物制药相关研究 |
| 英文名称 | Serpin A12 |
| 中文名称 | Serpin A12蛋白 |
| 单位 | 支 |
| 规格 | 10ug ; 50ug ; 500ug ; 1mg |
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文献和实验Expression of Recombinant Proteins with Uniform N-Termini
that recombinant proteins produced in this expression system contain homogeneous, unprocessed N-termini.
High Throughput Production of Recombinant Human Proteins for Crystallography
This chapter presents in detail the process used in high throughput bacterial production of recombinant human proteins for crystal structure determination. The core principles are: (1 ) Generating at least 10 truncated constructs
and centrally located DNA-binding domain (DBD), and a poorly characterized N-terminal domain that is required for maximal transcriptional activity (1 ,2 ) Human PR is expressed as two proteins from a single gene by alternate use of two promoters: PR
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