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- 详细信息
- 文献和实验
- 技术资料
- 库存:
现询
- 供应商:
北京索莱宝科技有限公司
- 规格:
1mg/500ug/50ug/10ug
| 规格: | 1mg | 产品价格: | ¥21120.0 |
|---|---|---|---|
| 规格: | 500ug | 产品价格: | ¥14784.0 |
| 规格: | 50ug | 产品价格: | ¥3276.0 |
| 规格: | 10ug | 产品价格: | ¥1092.0 |
| 基本信息 | |
| 基因名 | |
| 别名 | Ubiquitin-like modifier-activating enzyme 5;ThiFP1;UFM1-activating enzyme;Ubiquitin-activating enzyme E1 domain-containing protein 1;UBE1DC1;UBA5 |
| 外观(性状) | Supplied as a 0.2 μm filtered solution of 20mM TrisHCl,50mM NaCl,1mM DTT, 10% glycerol,pH 8.0 . |
| 纯度 | ≥95% as determined by reducing SDS-PAGE. |
| 储存条件 | Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles. |
| 运输条件 | 干冰运输 |
| 背景说明 | UBA5 is a member of the ubiquitin-activating E1 family and UBA5 subfamily.Ubiquitin and ubiquitin-like proteins are recognized as covalently conjugated to various cellular substrates by a three-step enzymatic pathway.The ubiquitin-activating enzyme (E1) has a vital role in the first step of ubiquitination pathway to activate ubiquitin or ubiquitin-like proteins.UBA5 activates ubiquitin-fold modifier 1,a ubiquitin-like post-translational modifier protein,via the formation of a high-energy thioester bond.UBA5 is located primarily in cytoplasm,while it generally localizes to the nucleus in presence of SUMO2. |
| 来源 | E.coli |
| 内毒素 | Less than 0.1 ng/ug(1 EU/ug)as determined by LAL test. |
| 单位 | 支 |
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文献和实验Studies of the Ubiquitin Proteasome System
and Purification of Anti‐Ubiquitin Antibodies Support Protocol 3: Preparation of Ubiquitin Affinity Column Generation of Reagent‐Grade Ubiquitin Activating Enzyme (E1) Basic Protocol 20: Preparation of Wheat E1 in E. coli Basic Protocol 21: Preparation of Mouse E1
Deubiquitinating Enzyme Purification, Assay Inhibitors, and Characterization
substrates to monitor DUB activity; and the purification of a recombinant human DUB, isopeptidase T, in E. coli using low-temperature expression as well as ion-exchange and affinity chromatography.
Proteasomes that Lack Endogenous USP14 and are Devoid of UB‐AMC Hydrolysis Activity Support Protocol 2: Purification of Recombinant Human USP14 from E. coli Support Protocol 3: Synthesis and Purification of Ubiquitin
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