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- Solarbio
- P00926
- 北京
- 2025年07月25日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
现询
- 供应商：
北京索莱宝科技有限公司
- 规格：
1mg/500ug/50ug/10ug
| 规格： | 1mg | 产品价格： | ￥15360.0 |
|---|---|---|---|
| 规格： | 500ug | 产品价格： | ￥10752.0 |
| 规格： | 50ug | 产品价格： | ￥2600.0 |
| 规格： | 10ug | 产品价格： | ￥936.0 |
| 基本信息 | |
| 基因名 | |
| 别名 | Activating receptor1;mAR-1;Lymphocyte antigen94;Naturalkiller cell p46-related protein;NK-p46;NKp46;mNKp46 |
| 外观(性状) | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 . |
| 纯度 | ≥95% as determined by reducing SDS-PAGE. |
| 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 运输条件 | The product is shipped at 2-8°C temperature. Upon receipt, store it immediately at the temperature listed below. |
| 背景说明 | Natural cytotoxicity triggering receptor 1(NKp46/NCR1) is a single-pass type I membrane protein.It consists of two extracellular Ig-like domains followed by a short stalk region，a transmembrane domain containing a positively charged amino acid residue，and a short cytoplasmictail.NKp46 is predominantly expressed in the embryo.It has a positive charge in its transmembrane domain that permits association with the ITAM-bearing signal adapter proteins，CD3 zeta and Fc epsilon RI gamma.These receptors are expressed almost exclusively by NK cells and play a major role in triggering some of the key lytic activities of NK cells.Studies with neutralizing antibodies indicate that the three NCR are primarily responsible for triggering the NK-mediated lysis of many human tumor celllines. |
| 来源 | Human Cells |
| 溶剂 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| 内毒素 | Less than 0.1 ng/μg(1 EU/μg)as determined by LAL test. |
| 单位 | 支 |
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文献和实验media (= RPMI 1640 + 10% FBS) PBS + 2% EDTA Triton X-100 solution (10% in dH2 O) Effector cell preparation: The mouse NK-cell line KY1 is used as an effector cell. During recovery (e.g. after freezing), these cells require 1000 U
R10 media (= RPMI 1640 + 10% FBS) PBS + 2% EDTA Triton X-100 solution (10% in dH2 O) Effector cell preparation: The mouse NK-cell line KY1 is used as an effector cell. During recovery (e.g. after freezing), these cells
High‐Throughput Cytotoxicity Screening by Propidium Iodide Staining
‐resolved analysis of individual wells. Cells were cultured 24 hr at 37°C in the presence of test compounds at a final concentration of 1 µM. Illustrated are cytotoxicity assay results from rows L through P (120 wells) of a 384‐well plate. Cell numbers
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