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- 文献和实验
- 技术资料
- 保质期:
Upon receipt, store it immediately at -20°C or lower for long term storage. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: <br>2-8°C for 2-7 days under sterile conditions after redissolves; <br>-20°C for 1 months and -70℃ for 3 months under sterile conditions after redissolves; <br>-20°C to -70℃ for 12 months in lyophilized state.
- 保存条件:
Upon receipt, store it immediately at -20°C or lower for long term storage. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: <br>2-8°C for 2-7 days under sterile conditions after redissolves; <br>-20°C for 1 months and -70℃ for 3 months under sterile conditions after redissolves; <br>-20°C to -70℃ for 12 months in lyophilized state.
- 英文名:
SIRPA
- 库存:
999
- 供应商:
北京索莱宝科技有限公司
- 规格:
10ug/50ug/500ug/1mg
| 规格: | 10ug | 产品价格: | ¥988.00 |
|---|---|---|---|
| 规格: | 50ug | 产品价格: | ¥2600.00 |
| 规格: | 500ug | 产品价格: | ¥14784.00 |
| 规格: | 1mg | 产品价格: | ¥21120.00 |
| 基因名 | SIRPA |
| 别名 | Tyrosine-Protein Phosphatase Non-Receptor Type Substrate 1; CD172 Antigen-Like Family Member A; Signal-Regulatory Protein Alpha-1; CD172a; SIRPA; MFR; SHPS1; SIRP |
| 蛋白号 | Q6P6I8 |
| 种属 | Mouse |
| 宿主 | HEK293 |
| 产品描述 | Recombinant Mouse Signal-Regulatory Protein Alpha 1 is produced by our Mammalian expression system and the target gene encoding Lys32-Asn373 is expressedwith a mouse IgG2a tag at the C-terminus. |
| 区间范围 | Lys32-Asn373 |
| 标签信息 | C-mIgG2aFc |
| 预测分子量 | 90-120 kDa |
| 表观分子量 | 90-120 KDa, reducing conditions |
| 纯度 | ≥95% by SDS‐PAGE. |
| 内毒素 | <1EU/ug as determined by LAL test. |
| 溶剂 | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
| 复溶 | Reconstituted protein solution can be diluted with distilled water. Always centrifuge tubes before opening. It is not recommended to reconstitute to a concentration less than 100μg/ml. |
| 储存条件 | Upon receipt, store it immediately at -20°C or lower for long term storage. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: 2-8°C for 2-7 days under sterile conditions after redissolves; -20°C for 1 months and -70℃ for 3 months under sterile conditions after redissolves; -20°C to -70℃ for 12 months in lyophilized state. |
| 运输条件 | 冷冻运输 |
| 背景说明 | SIRPα is a type I transmembrane glycoprotein.It contains two Ig-like C1-type domains and one Ig-like V-type domain. Mouse SIRP alpha ECD shares 61%, 75%, 62%, 61%, and 59% aa sequence identity with human, rat, equine, bovine, and porcine SIRP alpha, respectively.SIRPα can express in various tissues, mainly on brain and myeloid cells, including macrophages, neutrophils, dendritic and Langerhans cells. It also can detect in neurons, smooth muscle and endothelial cells. SIRPA is an immunoglobulin-like cell surface receptor for CD47. SIRPα acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPα shows adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. SIRPα engagement generally produces a negative regulatory signal; it may mediate negative regulation of phagocytosis, mast cell activation and dendritic cell activation. |
| 细胞活性 | No activity experimental data, to be verified. |
| ELISA活性 | No activity experimental data, to be verified. |
| SPR/BLI活性 | No activity experimental data, to be verified. |
| 研究领域 | 生物制药相关研究 |
| 英文名称 | SIRPA |
| 中文名称 | SIRPA蛋白 |
| 单位 | 支 |
| 规格 | 10ug ; 50ug ; 500ug ; 1mg |

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文献和实验with a Ca2+ -free medium or with trypsin/dispase. The latter facilitates the introduction of recombinant or trappable probes and microimaging studies of, for example, changes in cytosolic-free Ca2+ concentration or the dynamics of individual organelles
Purification of Recombinant Poly(ADP-Ribose) Polymerases
, more than 11 mg of pure and highly active mouse PARP-2 can be obtained from 1 L of Sf9 insect cell culture.
Rapid Isotyping of Mouse Monoclonal Antibodies
for 5% of mouse antibodies) has limited practical value. In contrast, the heavy-chain isotype is very informative (see Table 1 ); protein A or protein G binding, euglobulin properties, complement binding, kinetics of digestion with papain or pepsin, clearance
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