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- 文献和实验
- 技术资料
- 保质期：
Upon receipt, store it immediately at -20°C or lower for long term storage. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: <br>2-8°C for 2-7 days under sterile conditions after redissolves; <br>-20°C for 1 months and -70℃ for 3 months under sterile conditions after redissolves; <br>-20°C to -70℃ for 12 months in lyophilized state.
- 保存条件：
Upon receipt, store it immediately at -20°C or lower for long term storage. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: <br>2-8°C for 2-7 days under sterile conditions after redissolves; <br>-20°C for 1 months and -70℃ for 3 months under sterile conditions after redissolves; <br>-20°C to -70℃ for 12 months in lyophilized state.
- 英文名：
PD-L1
- 库存：
999
- 供应商：
北京索莱宝科技有限公司
- 规格：
10ug/50ug/500ug/1mg
| 规格： | 10ug | 产品价格： | ¥520.00 |
|---|---|---|---|
| 规格： | 50ug | 产品价格： | ¥1092.00 |
| 规格： | 500ug | 产品价格： | ¥6240.00 |
| 规格： | 1mg | 产品价格： | ¥10080.00 |
| 基因名 | PD-L1 |
| 别名 | Programmed cell death 1 ligand 1Cd274; programmed cell death 1 ligand 1; PD-L1; PDCD1 ligand 1; programmed death ligand 1; B7 homolog 1; B7-H1; CD274 |
| 蛋白号 | Q9EP73 |
| 种属 | Mouse |
| 宿主 | HEK293 |
| 产品描述 | Recombinant Mouse Programmed Cell Death 1 Ligand 1 is produced by our Mammalian expression system and the target gene encoding Phe19-Thr238 is expressed with a human IgG1 Fc tag at the C-terminus. |
| 区间范围 | Phe19-Thr238 |
| 标签信息 | C-Fc |
| 预测分子量 | 72-90 kDa |
| 表观分子量 | 72-90 KDa, reducing conditions |
| 纯度 | ≥95% by SDS‐PAGE. |
| 内毒素 | <1EU/ug as determined by LAL test. |
| 溶剂 | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH7.4. |
| 复溶 | Reconstituted protein solution can be diluted with distilled water. Always centrifuge tubes before opening. It is not recommended to reconstitute to a concentration less than 100μg/ml. |
| 储存条件 | Upon receipt, store it immediately at -20°C or lower for long term storage. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: 2-8°C for 2-7 days under sterile conditions after redissolves; -20°C for 1 months and -70℃ for 3 months under sterile conditions after redissolves; -20°C to -70℃ for 12 months in lyophilized state. |
| 运输条件 | 冷冻运输 |
| 背景说明 | Mouse Programmed cell death 1 ligand 1(Cd274, PD-L1), is a member of the growing B7 family of immune proteins.It involved in the costimulatory signal essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production.B7-H1 has been identified as one of two ligands for programmed death1 (PD1), a member of the CD28 family of immunoreceptors. B7-H1 is constitutively expressed in several organs such as heart, skeletal muscle B7-H1 expression is upregulated in a small fraction of activated T and B cells and a much larger fraction of activated monocytes. The costimulatory function of B7-H1 is critical for enhancing maturation and differentiation of T-cells in lymphoid organs.B7-H1 expression is also induced in dendritic cells and keratinocytes after IFN gamma stimulation. Interaction of B7-H1 with PD1 results in inhibition of TCR-mediated proliferation and cytokine production. The B7-H1:PD1 pathway is involved in the negative regulation of some immune responses and may play an important role in the regulation of peripheral tolerance. |
| 细胞活性 | No activity experimental data, to be verified. |
| ELISA活性 | Immobilized Mouse PD-L1-Fc at 2μg/ml (100μl/wel) can bind Anti-Human PDL1 mAb-Fc.The ED50 of Anti-Human PDL1 mAb-Fc is 9.72 ng/ml. |
| SPR/BLI活性 | No activity experimental data, to be verified. |
| 研究领域 | 生物制药相关研究 |
| 英文名称 | PD-L1 |
| 中文名称 | PD-L1蛋白 |
| 单位 | 支 |
| 规格 | 10ug ; 50ug ; 500ug ; 1mg |
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文献和实验depends on product and its function. N-terminal sequencing revealed that the purified rBoNTC(Hc )-N is missing the first eight amino acids of the N-terminus of the protein, whereas the purified rBoNTC(Hc )-C protein is intact. After a mouse bioassay test
11b/CD3/CD20/CD57 同时进行免疫荧光组化染色，并使用 Vectra 多光谱成像系统自动获取 8 通道 肿瘤组织芯片图像（下图 b），最后使用 InForm 定量分析软件，分别对 PD-L1 和另外 6 种免疫细胞标志物双阳性的免疫细胞群进行分析，发现肝癌组织中肿瘤区域内的 CD68 阳性巨噬细胞（Microphages, M φs）和肿瘤细胞（Tumor Cells, TCs）在 PD-L1 表达谱中具有最高的相关性（下图 c）。为研究 PD-L1 表达谱与巨噬细胞的相关性，文章中继续使用
Expression of Recombinant Cytochromes c in E. coli
for proper folding and stability. However, significant advances in expression of recombinant cytochromes c have been made during the last decade. It has been shown that a single gene cluster, ccmA–H , is responsible for cytochrome c maturation in Escherichia
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