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- 文献和实验
- 技术资料
- 保质期:
Upon receipt, store it immediately at -70°C or lower for long term storage. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: <br>2-8°C for 2-7 days; <br>-20°C for 1 months and -70℃ for 3 months.
- 保存条件:
Upon receipt, store it immediately at -70°C or lower for long term storage. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: <br>2-8°C for 2-7 days; <br>-20°C for 1 months and -70℃ for 3 months.
- 英文名:
CYPA
- 库存:
999
- 供应商:
北京索莱宝科技有限公司
- 规格:
10ug/50ug/500ug/1mg
| 规格: | 10ug | 产品价格: | ¥437 |
|---|---|---|---|
| 规格: | 50ug | 产品价格: | ¥1061 |
| 规格: | 500ug | 产品价格: | ¥6365 |
| 规格: | 1mg | 产品价格: | ¥9245 |
| 基因名 | CYPA |
| 别名 | Peptidyl-prolyl cis-trans isomerase A; PPIase A; Cyclophilin A; Cyclosporin A-binding protein; Rotamase A; SP18; PPIA; CYPA |
| 蛋白号 | P17742 |
| 种属 | Mouse |
| 宿主 | E.coli |
| 产品描述 | Recombinant Mouse Peptidyl-prolyl Cis-trans Isomerase A is produced by our E.coli expression system and the target gene encoding Met1-Leu164 is expressed. |
| 区间范围 | Met1-Leu164 |
| 标签信息 | Tag free |
| 预测分子量 | 16 kDa |
| 表观分子量 | 16 KDa, reducing conditions |
| 纯度 | ≥95% by SDS‐PAGE. |
| 内毒素 | <1EU/ug as determined by LAL test. |
| 溶剂 | Supplied as a 0.2 μm filtered solution of PBS, 10% Glycerol, pH 7.4. |
| 复溶 | Reconstituted protein solution can be diluted with distilled water. Always centrifuge tubes before opening. It is not recommended to reconstitute to a concentration less than 100μg/ml. |
| 储存条件 | Upon receipt, store it immediately at -70°C or lower for long term storage. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: 2-8°C for 2-7 days; -20°C for 1 months and -70℃ for 3 months. |
| 运输条件 | 干冰运输(需要收取干冰费) |
| 背景说明 | Peptidyl-prolyl cis-trans isomerase A is a cytoplasm protein which belongs to the cyclophilin-type PPIase family and PPIase A subfamily. Cyclophilins(CyPs) are a family of proteins found in organisms ranging from prokaryotes to humans. These molecules exhibit peptidyl-prolyl isomerase activity, suggesting that they influence the conformation of proteins in cells. Cyclophilin A accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Cyclophilin A can interact with several HIV proteins, including p55 gag, Vpr, and capsid protein, and has been shown to be necessary for the formation of infectious HIV virions. |
| 细胞活性 | No activity experimental data, to be verified. |
| ELISA活性 | No activity experimental data, to be verified. |
| SPR/BLI活性 | No activity experimental data, to be verified. |
| 研究领域 | 生物制药相关研究 |
| 英文名称 | CYPA |
| 中文名称 | CYPA蛋白 |
| 单位 | 支 |
| 规格 | 10ug ; 50ug ; 500ug ; 1mg |

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文献和实验with a Ca2+ -free medium or with trypsin/dispase. The latter facilitates the introduction of recombinant or trappable probes and microimaging studies of, for example, changes in cytosolic-free Ca2+ concentration or the dynamics of individual organelles
Purification of Recombinant Poly(ADP-Ribose) Polymerases
, more than 11 mg of pure and highly active mouse PARP-2 can be obtained from 1 L of Sf9 insect cell culture.
PRIMARY MOUSE KERATINOCYTE CULTURES Isolation of epidermal keratinocytes from neonatal mice is based on the protocol of Dlugosz et al., Methods Enzymol . 254:3-20 (1995). The epidermis from a newborn mouse should yield
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