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- 详细信息
- 文献和实验
- 技术资料
- 库存:
现询
- 供应商:
北京索莱宝科技有限公司
- 规格:
1mg/500ug/50ug/10ug
| 规格: | 1mg | 产品价格: | ¥21120.0 |
|---|---|---|---|
| 规格: | 500ug | 产品价格: | ¥14784.0 |
| 规格: | 50ug | 产品价格: | ¥4576.0 |
| 规格: | 10ug | 产品价格: | ¥1560.0 |
Recombinant Human beta-Actin is produced by our E.coli expression system and the target gene encoding Asp2-Phe375 is expressed with a 6His tag at the C-terminus.
| 基本信息 | |
| 基因名 | |
| 别名 | Actin Cytoplasmic 1;Beta-Actin;ACTB |
| 外观(性状) | Supplied as a 0.2 μm filtered solution of 10mM TrisHCl, 0.1% TritonX-100, 2mM DTT, pH 8.0. |
| 纯度 | ≥95% as determined by reducing SDS-PAGE. |
| 储存条件 | Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles. |
| 运输条件 | 干冰运输 |
| 背景说明 | Actins are ubiquitous globular and highly conserved proteins that are involved in various types of cell motility,structure,and integrity.Three main groups of actin isoforms,alpha,beta and gamma have been identified.The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus.The beta and gamma actins co-exist in most cell types as components of the cytoskeleton,and as mediators of internal cell motility.ACTB is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix.Each actin can bind to 4 others. |
| 来源 | E.coli |
| 内毒素 | Less than 0.1 ng/ug(1 IEU/ug)as determined by LAL test. |
| 单位 | 支 |
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文献和实验-actin呈现广谱且稳定的表达特征,几乎在所有哺乳动物的组织及细胞中持续表达,约占细胞总蛋白含量的50%,是细胞内表达量较高的基础功能蛋白之一。 数据库链接分享 SwissProt P60709 Human SwissProt P60710 Mouse SwissProt P60711 Rat 产品亮点 索莱宝开发的β-actin抗体具有广泛的物种交叉反应性和高度特异性,适用于多样的样本类型与研究场景。该抗体可稳定检测人、大鼠、小鼠的β-actin蛋白
Introduction of human 14-3-3 proteins
----------------------------------------------------------------------------A binding sequence for the 14-3-3 protein within the cytoplasmic domain of the adhesion receptor,platelet glycoprotein Ib alphaDu X; Fox JE; Pei SDepartment of Vascular Biology,Scripps Research Institute,La Jolla,California
【求助】新手 请问western-blot测NF-KB、FLT3是用总蛋白提取试剂盒吗
Anti-Flt-3, extracellular region,是针对flt3胞外段的抗体 Anti-Flt-3, cytoplasmic domain,是针对flt3胞内段的抗体 Flt-3 Ligand, recombinant human,是重组的Flt-3的配体,如果能作为抗体用的话应该是针对flt3的活性位点。不过如果是做western-blot的话,由于要蛋白要变性,变性后活性位点是否还存在不得而知,最好不要用这种,而且这种量少啊,才10 µg,估计比较贵吧。
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