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- 详细信息
- 文献和实验
- 技术资料
- 库存:
现询
- 供应商:
北京索莱宝科技有限公司
- 规格:
1mg/500ug/50ug/10ug
| 规格: | 1mg | 产品价格: | ¥21120.0 |
|---|---|---|---|
| 规格: | 500ug | 产品价格: | ¥14784.0 |
| 规格: | 50ug | 产品价格: | ¥3640.0 |
| 规格: | 10ug | 产品价格: | ¥1217.0 |
Recombinant Mouse Fms-like Tyrosine Kinase 3 Ligand is produced by our Mammalian expression system and the target gene encoding Gly27-Arg188 is expressed with a 6His tag at the C-terminus.
| 基本信息 | |
| 基因名 | |
| 别名 | Fms-related tyrosine kinase 3 ligand (Flt3L);SL cytokine;Flt3 ligand; |
| 外观(性状) | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 . |
| 纯度 | ≥95% as determined by reducing SDS-PAGE. |
| 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 运输条件 | The product is shipped at 2-8°C temperature. Upon receipt, store it immediately at the temperature listed below. |
| 背景说明 | Fms-related tyrosine kinase 3 ligand(Flt3L) is a single-pass type I membrane protein and consists of 232 amino acids.Flt3L is a hematopoietic four helical bundle cytokine,structurally homologous to stem cell factor and colony stimulating factor.Flt3L synergizes well with a number of other colony stimulating factors and interleukins.Flt3L stimulates the proliferation and differentiation of various blood cell progentiors by activating FLT3. |
| 来源 | Human cells |
| 溶剂 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| 内毒素 | Less than 0.1 ng/ug(1 IEU/ug)as determined by LAL test. |
| 单位 | 支 |
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文献和实验The Purification of Large Numbers of Antigen Presenting Dendritic Cells from Mouse Spleen
immunofluorescent labeling and flow cytometric cell sorting. If the availability of mice is limiting, our protocol can cater for DC numbers boosted by the administration of fms-like tyrosine kinase 3 ligand (Flt3L), directly via subcutaneous injection
Preparation of Recombinant Protein Spotted Arrays for Proteome‐Wide Identification of Kinase Targets
microarrays per kinase to be probed (e.g., from protocol 2 ) Superblock/0.1% Triton X‐100 (Thermo/Fisher) Kinase to be assayed
of many of these DC subpopulations from their precursors using bone marrow cultures supplemented with fms-like tyrosine kinase 3 ligand (Flt3L). The culture-generated DC can be aligned with the populations directly isolated from tissues. Combining the in vivo
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