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- 详细信息
- 文献和实验
- 技术资料
- 库存:
现询
- 供应商:
北京索莱宝科技有限公司
- 规格:
1mg/500ug/50ug/10ug
| 规格: | 1mg | 产品价格: | ¥21120.0 |
|---|---|---|---|
| 规格: | 500ug | 产品价格: | ¥14784.0 |
| 规格: | 50ug | 产品价格: | ¥3276.0 |
| 规格: | 10ug | 产品价格: | ¥1092.0 |
Recombinant Human Fms-like Tyrosine Kinase 3 Ligand is produced by our Mammalian expression system and the target gene encoding Thr27-Pro184 is expressed with a 6His tag at the C-terminus.
| 基本信息 | |
| 基因名 | |
| 别名 | Fms-Felated Tyrosine Kinase 3 Ligand;Flt3 Ligand;Flt3L;SL Cytokine;FLT3LG |
| 外观(性状) | Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0. |
| 纯度 | ≥95% as determined by reducing SDS-PAGE. |
| 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 运输条件 | The product is shipped at 2-8°C temperature. Upon receipt, store it immediately at the temperature listed below. |
| 背景说明 | Fms-Related Tyrosine Kinase 3 Ligand (FLT3LG) is a hematopoietic four helical bundle cytokine.Mature human Flt-3 Ligand consists of a extracellular domain (ECD) with a cytokine-like domain and a juxtamembrane tether region,a transmembrane segment,and a cytoplasmic tail.Human and mouse Flt-3 Ligand show cross-species activity.Flt-3 Ligand is expressed as a noncovalently-linked dimer by T cells and bone marrow and thymic fibroblasts.It is structurally homologous to stem cell factor (SCF) and colony stimulating facor 1 (CSF-1).In synergy with other growth factors,Flt3 ligand stimulates the proliferation and differentiation of various blood cell progenitors by activiation of Flt 3 receptor. |
| 来源 | Human cells |
| 溶剂 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| 内毒素 | Less than 0.1 ng/ug(1 EU/ug)as determined by LAL test. |
| 单位 | 支 |
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文献和实验Preparation of Recombinant Protein Spotted Arrays for Proteome‐Wide Identification of Kinase Targets
microarrays per kinase to be probed (e.g., from protocol 2 ) Superblock/0.1% Triton X‐100 (Thermo/Fisher) Kinase to be assayed
of many of these DC subpopulations from their precursors using bone marrow cultures supplemented with fms-like tyrosine kinase 3 ligand (Flt3L). The culture-generated DC can be aligned with the populations directly isolated from tissues. Combining the in vivo
Introduction of human 14-3-3 proteins
whose members have been shown to activate tyrosine and tryptophan hydroxylases,inhibit protein kinase C and possess phospholipase A2 activity in vitro.We have isolated and analyzed a 14-3-3 protein cDNA clone (H14-3-3)from a human fetal brain cDNA library
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