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- 文献和实验
- 技术资料
- 库存：
现询
- 供应商：
北京索莱宝科技有限公司
- 规格：
1mg/500ug/50ug/10ug
| 规格： | 1mg | 产品价格： | ￥21120.0 |
|---|---|---|---|
| 规格： | 500ug | 产品价格： | ￥14784.0 |
| 规格： | 50ug | 产品价格： | ￥4576.0 |
| 规格： | 10ug | 产品价格： | ￥1560.0 |
Recombinant Human Matrix Metalloproteinase-2 is produced by our Mammalian expression system and the target gene encoding Ala30-Cys660 is expressed with a 6His tag at the C-terminus.
【本资料源自公开渠道，如需（此处）屏蔽，请联系删除】
标题:In vivo self-assembled bispecific fluorescence probe for early detection of bladder cancer and metastasis
成员:Da-Yong Hou, Ni-Yuan Zhang, Peng Zhang, Xiang-Peng Li, Jiong-Cheng Wu, Mei-Yu Lv, Zhi-Jia Wang, Xing-Jie Hu, Jian-Xiao Liang, Hong-Lei Wang, Yue-Ze Wang, Hui-Hui You, Hong-Wei An, Hao Wang, Wanhai Xu
论文因子:18.8 发表期刊:Science Bulletin pmid:39537458
| 基本信息 | |
| 基因名 | |
| 别名 | 72 kDa Type IV Collagenase;72 kDa Gelatinase;Gelatinase A;Matrix Metalloproteinase-2;MMP-2;TBE-1;MMP2;CLG4A |
| 外观(性状) | Note: The proenzyme needs to be activated by APMA. Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 7.5. |
| 纯度 | ≥95% as determined by reducing SDS-PAGE. |
| 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 运输条件 | The product is shipped at 2-8°C temperature. Upon receipt, store it immediately at the temperature listed below. |
| 背景说明 | 72 kDa type IV collagenase also known as matrix metalloproteinase-2 (MMP-2) and gelatinase A is an enzyme that in humans is encoded by the MMP2 gene.It belongs to the matrix metalloproteinase (MMP) family.Matrix metalloproteinases (MMPs) are a family of zinc-dependent endopeptidases that degrade components of the extracellular matrix (ECM) and play essential roles in various physiological processes such as morphogenesis，differentiation，angiogenesis and tissue remodeling，as well as pathological processes including inflammation，arthritis，cardiovascular diseases，pulmonary diseases and tumor invasion.MMP-2 is ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature，angiogenesis，tissue repair，tumor invasion，inflammation，atherosclerotic plaque rupture，as well as degrading extracellular matrix proteins.MMP-2 can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction.MMP-2 cleaves KISS at a Gly-|-Leu bond and appears to have a role in myocardial cell death pathways. |
| 来源 | Human cells |
| 溶剂 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| 内毒素 | Less than 0.1 ng/ug(1 EU/ug)as determined by LAL test. |
| 单位 | 支 |
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文献和实验【本资料源自公开渠道，如需（此处）屏蔽，请联系删除】
标题:In vivo self-assembled bispecific fluorescence probe for early detection of bladder cancer and metastasis
成员:Da-Yong Hou, Ni-Yuan Zhang, Peng Zhang, Xiang-Peng Li, Jiong-Cheng Wu, Mei-Yu Lv, Zhi-Jia Wang, Xing-Jie Hu, Jian-Xiao Liang, Hong-Lei Wang, Yue-Ze Wang, Hui-Hui You, Hong-Wei An, Hao Wang, Wanhai Xu
论文因子:18.8 发表期刊:Science Bulletin pmid:39537458
-1 Collagenase-1 Collagens I, II, III, VI, X, gelatins, aggrecan, entactin (154–158) MMP-2 72
Introduction of human 14-3-3 proteins
of the tryptic peptides derived from the purified enzyme revealed two amino acid sequences homologous to the 14-3-3 proteins.A polyclonal antibody was raised against purified ST-IV.A 33 kDa protein was co-immunoprecipitated from rat brain extracts with the anti
在无血清细胞培养条件下将人 iPS 细胞体外分化为结肠类器官
-1000 移液器上下吹打 20 次以分散类器官沉淀团。 向类器官悬浮液中加入 5 mL 1X PBS，并在 4 °C 下以 700 x g 离心 5 分钟。如果上清液中存在残留的类器官碎片，则再离心 5 分钟。 吸出上清液并用 1 mL 3dGRO™ 类器官冷冻培养基 (SCM301) 重悬沉淀。 用 P-1000 移液器重悬沉淀 5 次，加入冷冻培养基以获得四个胶滴每毫升每管 将冷冻保存管中的细胞转移到冷冻容器（例如 Mr. Frosty）中，然后放入 -80 °C 冰箱中 24-72 小时
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