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- 文献和实验
- 技术资料
- 库存：
现询
- 供应商：
北京索莱宝科技有限公司
- 规格：
1mg/500ug/50ug/10ug
| 规格： | 1mg | 产品价格： | ￥2080.0 |
|---|---|---|---|
| 规格： | 500ug | 产品价格： | ￥1456.0 |
| 规格： | 50ug | 产品价格： | ￥520.0 |
| 规格： | 10ug | 产品价格： | ￥260.0 |
Recombinant Human Leptin is produced by our E.coli expression system and the target gene encoding Val22-Cys167 is expressed.
【本资料源自公开渠道，如需（此处）屏蔽，请联系删除】
标题:Effects and mechanisms of Xiaochaihu Tang against liver fibrosis: An integration of network pharmacology, molecular docking and experimental validation
成员:Shou-Jia Wang, Wen Ye, Wan-Yi Li, Wen Tian, Meng Zhang, Yang Sun, Ying-Da Feng, Chen-Xu Liu, Shao-Yuan Liu, Wei Cao, Jing-Ru Meng, Xiao-Qiang Li
论文因子:5.195 发表期刊:JOURNAL OF ETHNOPHARMACOLOGY pmid:36529247
【本资料源自公开渠道，如需（此处）屏蔽，请联系删除】
标题:Diet containing stearic acid increased food intake in mice by reducing serum leptin compared with oleic acid
成员:Shuqing Zhu, Xiaohua Feng, Xiajie Feng, Kailai Xie, Yongxiang Li, Lvshuang Chen, Yingfen Mo, Jingwen Liang, Xin Wu, Zhonghua Sun, Gang Shu, Songbo Wang, Ping Gao, Xiaotong Zhu, Canjun Zhu, Qingyan Jiang, Lina Wang
论文因子:6.317 发表期刊:Food & Function pmid:36545693
| 基本信息 | |
| 基因名 | |
| 别名 | Leptin;Obese Protein;Obesity Factor;LEP;OB;OBS |
| 外观(性状) | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
| 纯度 | ≥95% as determined by reducing SDS-PAGE. |
| 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 运输条件 | The product is shipped at 2-8°C temperature. Upon receipt, store it immediately at the temperature listed below. |
| 背景说明 | Leptin is a hormone secreted from white adipocytes and plays important role in the regulation of food intake and energy balance.Leptin functions via signaling pathways involving OB-R in hypothalamus.Animal models have revealed the influence of Leptin in reducing body weight and regulating blood glucose level.When mutations are introduced in obese gene，mice with impaired function of leptin are massively obese and in high risk of diabetes.Leptin deficiency reduces metablic rate.Leptin deficient mice are less active and with lower body temperature than normal animals.Human Leptin shares approximately 84% sequence identity with the mouse protein.Human Leptin consists of 167 amino acid residue including a 21 amino acid residue signal sequence and 146 amino acid residue mature protein sequence. |
| 来源 | E.coli |
| 溶剂 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| 内毒素 | Less than 0.1 ng/ug(1 EU/ug)as determined by LAL test. |
| 单位 | 支 |
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文献和实验【本资料源自公开渠道，如需（此处）屏蔽，请联系删除】
标题:Effects and mechanisms of Xiaochaihu Tang against liver fibrosis: An integration of network pharmacology, molecular docking and experimental validation
成员:Shou-Jia Wang, Wen Ye, Wan-Yi Li, Wen Tian, Meng Zhang, Yang Sun, Ying-Da Feng, Chen-Xu Liu, Shao-Yuan Liu, Wei Cao, Jing-Ru Meng, Xiao-Qiang Li
论文因子:5.195 发表期刊:JOURNAL OF ETHNOPHARMACOLOGY pmid:36529247
【本资料源自公开渠道，如需（此处）屏蔽，请联系删除】
标题:Diet containing stearic acid increased food intake in mice by reducing serum leptin compared with oleic acid
成员:Shuqing Zhu, Xiaohua Feng, Xiajie Feng, Kailai Xie, Yongxiang Li, Lvshuang Chen, Yingfen Mo, Jingwen Liang, Xin Wu, Zhonghua Sun, Gang Shu, Songbo Wang, Ping Gao, Xiaotong Zhu, Canjun Zhu, Qingyan Jiang, Lina Wang
论文因子:6.317 发表期刊:Food & Function pmid:36545693
Secretion of Recombinant Human Insulin-Like Growth Factor I (IGF-I)
The development of efficient recombinant protein production processes can be a critical factor in whether or not a pharmaceutical therapeutic protein can enter human clinical trials and ultimately the marketplace. This is especially true
In this chapter we describe efficient procedures for the construction, expression and screening of comprehensive libraries of human Fab antibody fragments displayed on the surface of filamentous phage. Phagemid vectors are used for placing
我们知道，PeproTech的所有细胞因子和蛋白均为冻干粉，这使得运输非常便捷，只要常温即可。而且，细胞因子和蛋白冻干粉非常稳定，在-20℃或-80℃条件下可保存数年。冻干粉在使用前需进行溶解，然后以液体形式加到培养体系或注射入动物体内。溶解步骤非常关键，因溶解不好会导致细胞因子或蛋白的失活，这也是很多用户在实际使用中经常遇到的问题。 那么，应该如何进行正确的溶解呢？ 下面我们以Recombinant Human IL-4 (重组人IL-4，产品编号：200-04)的说明书为例
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