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- Solarbio
- 北京
- G7951
- 2026年08月13日
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- 文献和实验
- 技术资料
- 保质期：
Upon receipt, store it immediately at -70°C or lower for long term storage. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: <br>2-8°C for 2-7 days; <br>-70℃ for 1 months.
- 保存条件：
Upon receipt, store it immediately at -70°C or lower for long term storage. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: <br>2-8°C for 2-7 days; <br>-70℃ for 1 months.
- 英文名：
GFP
- 库存：
999
- 供应商：
北京索莱宝科技有限公司
- 规格：
50ug
| 基因名 | GFP |
| 别名 | GFP |
| 蛋白号 | P42212 |
| 种属 | Water jellyfish |
| 宿主 | E.coli |
| 产品描述 | Recombinant GFP 重组绿色荧光蛋白 is produced by our E.coli expression system and the target gene encoding 1-238aa is expressed with a N-His tag. |
| 区间范围 | 1-238aa |
| 标签信息 | N-His |
| 预测分子量 | 26.07 kDa |
| 表观分子量 | 26 kDa |
| 纯度 | ≥90% by SDS-PAGE. |
| 内毒素 | Not measured |
| 溶剂 | 20mM Tris-HCl（PH8.0）with protectants（50mM NaCl, 1mM EDTA, 5% trehalose, 10% Glycerol) |
| 复溶 | Reconstituted protein solution can be diluted with distilled water. Always centrifuge tubes before opening. It is not recommended to reconstitute to a concentration less than 100μg/ml. |
| 储存条件 | Upon receipt, store it immediately at -70°C or lower for long term storage. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: 2-8°C for 2-7 days; -70℃ for 1 months. |
| 运输条件 | 干冰运输（需要收取干冰费） |
| 背景说明 | Recombinant GFP 重组绿色荧光蛋白 is a functionally important regulatory protein involved in diverse cellular biological processes, playing a critical role in maintaining cellular homeostasis and regulating key physiological functions. It is widely expressed in various mammalian tissues and cell types, and participates in multiple essential signaling pathways and molecular mechanisms that control cell proliferation, differentiation, apoptosis, metabolism, and immune responses. This protein has been extensively studied in the context of biomedical research, with its dysregulation linked to numerous pathological conditions including cancer, inflammatory disorders, metabolic diseases, and neurodegenerative conditions. As a high-quality recombinant protein, it is widely used in in vitro functional assays, protein-protein interaction studies, drug development and screening, and basic biological research, providing a reliable and essential tool for investigating its molecular mechanisms, biological functions, and therapeutic potential in various disease contexts. |
| 细胞活性 | No activity experimental data, to be verified. |
| ELISA活性 | No activity experimental data, to be verified. |
| SPR/BLI活性 | No activity experimental data, to be verified. |
| 研究领域 | 荧光标记与成像 |
| 英文名称 | GFP |
| 中文名称 | GFP蛋白 |
| 单位 | 支 |
| 规格 | 50ug |
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文献和实验dmdfe 本人将GFP基因后面加了一段大约110bp的序列，插入PET28A的BamH1和Hind3位点，最后在BL21中表达。 经SDS-PAGE验证表明： 目的蛋白 大量存在菌体裂解后沉淀即包涵体中，但是该沉淀无色。 少量存在于上清中，上清显微弱的绿色。 看文献大量报道GFP融合蛋白几乎都形成可溶性表达，目的蛋白在上清中，并且诱导条件没有太大要求。 想请教一下各位做过GFP原核表达的经验
一、 GFP背景概述 绿色荧光蛋白（green fluorescent protein, GFP）最早由Osamu Shimomura于1962年在水母（Aequorea victoria）（图2a）中发现。这种蛋白质在蓝色波长范围的光照激发下发出绿色荧光，其发光过程需要冷光蛋白质Aequorin的帮助，而且，这个冷光蛋白质可与钙离子（Ca2+）相互作用（图3）。在Aequorea victoria中发现的野生型绿色荧光蛋白的分子量较小，仅为27~30kDa，而编码GFP的基因序列也很短
一、实验目的 1.了解绿色荧光蛋白和共聚焦扫描显微镜在细胞分子生物学中的应用 2.学会使用质膜蛋白的分析软件。 二、带GFP的质膜蛋白分选概述 1．质膜蛋白的跨膜信号 在粗面内质网上合成的蛋白质有两类：分泌蛋白和膜蛋白，分泌蛋白在内质网上合成之后通过多种信号切除后释放到内质网的腔中，进行下游途径的转运。膜蛋白较为复杂，它要靠疏水区滞留在内质网膜上。下面以酵母二价
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