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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Powder:2-8℃，2 years;Insolvent(母液):-20℃，6 months;-80℃，1 year
- 保质期：
Powder:2-8℃，2 years;Insolvent(母液):-20℃，6 months;-80℃，1 year
- 英文名：
AX20017
- 库存：
现询
- 供应商：
北京索莱宝科技有限公司
- CAS号：
329221-38-7
- 规格：
50mg/25mg/10mg/5mg
| 规格： | 50mg | 产品价格： | ￥2240.0 |
|---|---|---|---|
| 规格： | 25mg | 产品价格： | ￥1390.0 |
| 规格： | 10mg | 产品价格： | ￥690.0 |
| 规格： | 5mg | 产品价格： | ￥490.0 |
| 基本信息 | |
| CAS | No.329221-38-7 |
| 英文名称 | AX20017 |
| 分子式 | C13H16N2O2S |
| 分子量 | 264.34 |
| 溶解性 | Soluble in DMSO ≥5mg/mL(Need ultrasonic) |
| 纯度 | ≥98% |
| 外观(性状) | White to off-white Solid |
| 储存条件 | Powder:2-8℃，2 years;Insolvent(母液):-20℃，6 months;-80℃，1 year |
| MDL | MFCD00785286 |
| SMILES | C1CCC2=C(C1)C(=C(S2)NC(=O)C3CC3)C(=O)N |
| InChIKey | VATFNEMGBRWLHI-UHFFFAOYSA-N |
| InChI | InChI=1S/C13H16N2O2S/c14-11(16)10-8-3-1-2-4-9(8)18-13(10)15-12(17)7-5-6-7/h7H，1-6H2，(H2，14，16)(H，15，17) |
| PubChem CID | 673481 |
| 靶点 | protein kinase G (PknG) |
| 通路 | Metabolic Enzyme&Protease |
| 背景说明 | AX20017是protein kinase G (PknG)的小分子抑制剂。 |
| 生物活性 | AX20017 is a small-molecule protein kinase G (PknG) inhibitor with an IC50 of 0.39 μM.[1] |
| IC50 | IC50: 0.39 μM (PknG)[1] |
| In Vitro | The compound AX20017 inhibitor is bound deep within a narrow pocket formed by the inter lobe cleft of the PknG domain. The main chain Glu233:O and Val235:NH of PknG form hydrogen bonds with AX20017[2].AX20017 results in mycobacterial transfer to lysosomes and killing of the mycobacteria. AX20017 does not affect the human kinases， whereas the activity of PknG is effectively inhibited. AX20017 does not affect cellular morphology， membrane ruffling， or macropinocytosis[3]. |
| 细胞实验 | Phagocytosis is analyzed after incubation of J774 cells for 30 min in the presence of the indicated concentration of AX20017 (0， 10， 20 μM)， followed by incubating the cells for 2 h with latex beads at a ratio of 10:1 beads/cells in the continued presence of the inhibitor， followed by fixation in 3% paraformaldehyde as described. Cells are observed with a Axiophot using a ×63 objective. Proliferation of J774 cells is analyzed by incorporation of tritiated thymidine (0.1 μCi) for 12 h as described of cells that had been incubated for 48 h in the absence or presence of the AX20017(0， 10， 20 μM)[3]. |
| 激酶实验 | In vitro phosphorylation by PknG (0.5 μg) is in 25 mM Tris (pH 7.5)， 2 mM MnCl2， and 0.5 μCi [γ-32P]ATP in the absence or presence of the reagents. To monitor kinase activity of PknGΔN， the protein is combined with equal amounts of the kinase-dead mutant of full-length PknG， PknG-K181M. To analyze kinase activity of PknG-I87S/A92S and PknG-C/S， the PknG-N-terminal fragment of PknG (2 μg) is included. Phosphorylated proteins are separated on 12.5% SDS/PAGE and analyzed by autoradiography or quantitated by PhosphorImage analysis. IC50 values are determined by using a radiometric ATP consumptive assay. Twelve concentrations of AX20017 in the range from 5 × 10-5M to 1.5 × 10-10 M are tested in each kinase assay[3]. |
| 数据来源文献 | [1]. Walburger A， et al. Protein kinase G from pathogenic mycobacteria promotes survival within macrophages. Science. 2004 Jun 18;304(5678):1800-4. [2]. Santhi N， et al. Insights from the molecular docking of withanolide derivatives to the target protein PknG from Mycobacterium tuberculosis. Bioinformation. 2011;7(1):1-4. [3]. Scherr N， et al. Structural basis for the specific inhibition of protein kinase G， a virulence factor of Mycobacterium tuberculosis. Proc Natl Acad Sci U S A. 2007 Jul 17;104(29):12151-6. |
| 单位 | 瓶 |
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文献和实验磷酰胺（DEPC) (Sigma) 、30% 甘油及蛋白酶抑制剂 [ 含有 1 μg/ml 抑肽酶（aprotinin) 、1 μg/ml 胃蛋白酶抑制剂（pepstatin) 、1 μg/ml 亮抑酶肽（leupeptin) 、0.1 mmol/L PMSF ] ，所有药品都来自于 Sigma ( 见注释 1 和注释 2) 。注意：媒介中的两种成分—— DEPC 和 β-巯基乙醇是有毒的，需要在通风橱中进行准备，并需要戴化学防护手套。 2.2 细胞核的提纯 ( 1 ) 样品
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