IC4870 刀豆蛋白A 抑制剂/拮抗剂/激动剂 索莱宝

使用本产品的案例（仅供参考）

In Vitro

Lymphocyte（淋巴细胞，2.5 μg/ml ConA ）

Purified lymphocytes were collected by centrifugation (1800 r,10 min). Lymphocytes were suspended with RPMI 1640 medium containing 4% fetal bovine serum, 2.5 μg/ml ConA (Beijing Solarbio Science Technology Co., Ltd., Beijing, China) and seeded in 6-well plates at a density of 2 × 106 cells/mL 

来源文献：Ma Y, Ye Y, Liu Y, Chen J, Cen Y, Chen W, Yu C, Zeng Q, Zhang A, Yang G. DNMT1-mediated Foxp3 gene promoter hypermethylation involved in immune dysfunction caused by arsenic in human lymphocytes. Toxicol Res (Camb). 2020 Aug 6;9(4):519-529. doi: 10.1093/toxres/tfaa056. PMID: 32905139; PMCID: PMC7467266.

T/B lymphocyte（T淋巴细胞，4μg/ml Con A）

The proliferation of spleen T/B lymphocyte was also determined using an MTT assay as above. Briefly, 100 μl of cell suspension was seeded in a well of 96-well culture plates. After the addition of 50 μl Con A (16 μg/mL) or LPS (4 μg/mL) and 50μL COS-Se (80, 200, 400, 800 and 2000 μg/mL), the plates were incubated for 48 h at 37°C in a 5% humidified CO2. The stimulation index (SI/%) was calculated using the following equation: 

SI (%) = Absorbance of COS-Se groups/Absorbance of Con A or LPS groups × 100%.

来源文献：Jiang Z, Chi J, Li H, Wang Y, Liu W, Han B. Effect of chitosan oligosaccharide-conjugated selenium on improving immune function and blocking gastric cancer growth. Eur J Pharmacol. 2021 Jan 15;891:173673. doi: 10.1016/j.ejphar.2020.173673. Epub 2020 Oct 22. PMID: 33098836.

Lymphocyte（淋巴细胞，10 μg/mL ConA）

Within 24 h of the last administration, the mice were sacrificed in a sterile environment. Spleen tissue was disrupted, and spleen cell suspensions were passed through sterile nylon mesh. Red blood cells were lysed by Erythrocyte Lysate (Solarbio, China). Cells were resuspended in RPMI 1640 medium (Solarbio, China) at the concentration of 5 x 10⁶ cells/L. Blastogenic response of splenocytes to the mitogens, concanavalin A (ConA, Solarbio, China), and lipopolysaccharide (LPS, Solarbio, China), were assessed by CCK-8. Splenocytes suspension was incubated with ConA (10 μg/mL) or LPS (15 μg/mL) in 150μL RPMI 1640 medium containing 10% foetal bovine serum at 37 °C with 5% CO2. After incubation for 44 h, 10 lL CCK-8 was added to each well. After incubation for 2 h, the absorbance at 450 nm was measured by a microplate reader.

来源文献：Chen M, Fu Q, Song X, Muhammad A, Jia R, Zou Y, Yin L, Li L, He C, Ye G, Lv C, Liang X, Huang J, Cui M, Yin Z. Preparation of resveratrol dry suspension and its immunomodulatory and anti-inflammatory activity in mice. Pharm Biol. 2020 Dec;58(1):8-15. doi: 10.1080/13880209.2019.1699123. PMID: 31847682; PMCID: PMC6968662.

In Vivo

Mice（Female C57BL/6 mice (6-week-old)，15 mg/kg ConA ，iv. ）

All the mice were acclimatized for 1 week and then randomized into four groups (n=6-7 per group). According to the previous studies mice were intravenously (i.v.) injected with ConA (15 mg/kg; Solarbio, Beijing, China) for 12 h to establish a mouse model of IMH. 

来源文献：Liu M, Zhang H, Zhang L, Liu X, Zhou S, Wang X, Zhong W, Zhang J, Wang B, Zhao J, Zhou L. RIP3 blockade prevents immune-mediated hepatitis through a myeloid-derived suppressor cell dependent mechanism. Int J Biol Sci. 2022 Jan 1;18(1):199-213. doi: 10.7150/ijbs.65402. PMID: 34975327; PMCID: PMC8692153.

In Vitro( mouse lymphocyte )

The separated lymphocytes were cultured with a final concentration of 0, 24.57 ± 0.55, and 100.56 ± 1.48 μg/mL LCNP or 1 μg/mL ConA for 48 h Subsequently, MTT was added, and the absorbance at 570 nm in each well was determined using a microplate reader.

Lymphocytes were seeded into 96-well plates at 5.23 ± 0.22 × 105 cells/well containing complete RPMI 1640 medium and treated with a concentration of 0, 24.57 ± 0.55, or 100.56 ± 1.48 μg/mL LCNP and 1.05 ± 0.13 μg/mL ConA.

Lymphocytes were cultured in a 24-well plate at 2.23 ± 0.28 × 106 cells/well with final concentrations of 0, 24.57 ± 0.55, or 100.56 ± 1.48 μg/mL LCNP and 1 μg/mL ConA. 

来源文献：Zhou Y F, Nie J, Sun J X, et al. A branched galactoglucan from Lysimachia christinae: Structural characterization, immunoregulatory and anti-oxidant bioactivities in vitro and in vivo[J]. Carbohydrate Polymer Technologies and Applications, 2025, 9: 100653.

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标题:Effect of chitosan oligosaccharide-conjugated selenium on improving immune function and blocking gastric cancer growth

成员:JiangZ;ChiJ;LiH;WangY;LiuW;HanB

论文因子:4.12 发表期刊:Eur J Pharmacol pmid:33098836

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标题:A novel polysaccharide from Castanea mollissima Blume: Preparation; characteristics and antitumor activities in vitro and in vivo

成员:Dong XD;Feng YY;Liu YN;Ji HY;Yu SS;Liu A;Yu J.

论文因子:6.044 发表期刊:Carbohydrate Polymers pmid:32475583

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标题:The effective approach for the quality control of Codonopsis radix based on quality markers of immune activity.

成员:Liu J, Wang Y, Li B, Qi Y, Wei X, Liu H, Zhang B,

论文因子:3.337 发表期刊:J Sep Sci. pmid:35138677

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标题:RIP3 blockade prevents immune-mediated hepatitis through a myeloid-derived suppressor cell dependent mechanism.

成员:Liu M, Zhang H, Zhang L, Liu X, Zhou S, Wang X, Zh

论文因子:6.087 发表期刊:Int J Biol Sci. pmid:34975327

【本资料源自公开渠道，如需（此处）屏蔽，请联系删除】

标题:A novel polysaccharide from Castanea mollissima Blume: Preparation, characteristics and antitumor activities in vitro and in vivo.

成员:Dong XD, Feng YY, Liu YN, Ji HY, Yu SS, Liu A, Yu

论文因子:6.044 发表期刊:Carbohydr Polym. pmid:32475583

基本信息
CAS	No.11028-71-0
中文名称	刀豆蛋白A
英文名称	Concanavalin A
别名	CRD-401;刀豆凝集素A;伴刀豆球蛋白A;伴刀豆凝集素;刀豆素A;Knife bean protein A
溶解性	Soluble in Water ≥10mg/mL
纯度	≥90%
来源	Canavalia ensiformis
外观(性状)	White to off-white Solid
储存条件	Powder:-20℃，2 years;Insolvent(母液):-20℃，6 months;-80℃，1 year
EC	EINECS 234-258-2
MDL	MFCD00071069
SMILES	CCCN(CCC)S(=O)(=O)C1=CC=C(C=C1)C(=O)O.CC1=CN(C(=O)NC1=O)C2CC(C(O2)CO)N=[N+]=[N-]
InChIKey	RVSZVJZFCQZYHQ-UHFFFAOYSA-N
InChI	InChI=1S/C13H19NO4S.C10H13N5O4/c1-3-9-14(10-4-2)19(17,18)12-7-5-11(6-8-12)13(15)16;1-5-3-15(10(18)12-9(5)17)8-2-6(13-14-11)7(4-16)19-8/h5-8H,3-4,9-10H2,1-2H3,(H,15,16);3,6-8,16H,2,4H2,1H3,(H,12,17,18)
PubChem CID	155486958
靶点	Others
通路	Others
背景说明	是一种植物凝集素,具有促进有丝分裂的活性，有较好的促淋巴细胞转化作用，主要对T淋巴细胞有激发作用。
单位	瓶