- ￥6250
- Indoor Biotechnologies
- 美国
- RP-GM4-1
- 2025年07月21日
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- 详细信息
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- 库存：
1000
- 供应商：
北京博蕾德生物科技有限公司
- 规格：
250µg
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文献和实验Recombinant expression and SDS
is obtained. 12.The TA will take the gel pictures. SDS -PAGE sample buffer (2X) 0.125 M Tris-CL pH 6.8 4% SDS 20% glycerol 2% 2-mercaptoethanol 0.01% bromphenol blue Acrylamide Solutions for Two Laemmli
DETECTION OF RECOMBINANT PROTEINS BY ELISA
of interest. If the recombinant protein is not soluble in H2 O, dissolve the insoluble pellet in a volume of 7 M urea equal to 1/10 of the bacterial culture volume. Vortex the samples at a medium speed for about 30 minutes (use a vortex setting of 4.5 on the Fisher Vortex Genie
Nonradioactive Labeling and lmmunodetection of Recombinant Proteins
Recombinant proteins can be detected and characterized via several immunodetection schemes. Native proteins are typically detected by applying them to a membrane manually or using a vacuum manifold in a dot-or slot-blot format. This approach
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