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Sigma货号W1878基础培养液(Williams’Med

ium E)上海睿安生物13611631389
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  • ￥742.07
  • Sigma-Aldrich
  • made in USA
  • W1878
  • 2025年11月18日
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    上海睿安生物科技有限公司
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    • 文献和实验
    • 技术资料
    • 保存条件：

      2-8°C

    • 保质期：

      1年

    • 英文名：

      Williams’ E medium

    • 库存：

      100⁺瓶

    • 供应商：

      上海睿安生物13611631389

    • 规格：

      500ml/瓶

    Sigma货号W1878-500ml基础培养液(Williams’Medium E)上海睿安生物13611631389

     

    Sigma货号W1878-6✕500ml基础培养液(Williams’Medium E)上海睿安生物13611631389

    Williams′ 培养基E

    With sodium bicarbonate,without ʟ-glutamine and phenol red,liquid,sterile-filtered,suitable for cell culture

    别名:Williams’ E medium

    说明

    一般描述:Williams培养基E由Williams和Gunn在Williams培养基D的基础上改良而成。培养基专用于支持成人肝脏上皮细胞的长期细胞培养。它是改良型最低必需培养基(MEM)，改变了葡萄糖和氨基酸的含量。

    应用:Williams培养基E已用于培养人肝细胞癌(HCC)细胞和肝细胞。

    重悬:添加0.292g/L L-谷氨酰胺。

    其他说明:酚红会干扰一些细胞在低密度或克隆密度下的生长。在使用干细胞或低密度细胞时，请使用这款William's E。

    产品细节图片1

    同行评审论文(部分文献)

    Metabolic Profiling of Human Long-Term Liver Models and Hepatic Clearance Predictions from In Vitro Data Using Nonlinear Mixed-Effects Modeling.
    Nicole A Kratochwil et al.
    The AAPS journal, 19(2), 534-550 (2017-01-05)
    Early prediction of human clearance is often challenging, in particular for the growing number of low-clearance compounds. Long-term in vitro models have been developed which enable sophisticated hepatic drug disposition studies and improved clearance predictions. Here, the cell line HepG2
    Impact of Environmental Chemicals on the Transcriptome of Primary Human Hepatocytes: Potential for Health Effects.
    Robert D Mitchell et al.
    Journal of biochemical and molecular toxicology, 30(8), 375-395 (2016-04-20)
    New paradigms for human health risk assessment of environmental chemicals emphasize the use of molecular methods and human-derived cell lines. In this study, we examined the effects of the insect repellent DEET (N,N-diethyl-m-toluamide) and the phenylpyrazole insecticide fipronil (fluocyanobenpyrazole) on
    Chromatin recruitment of activated AMPK drives fasting response genes co-controlled by GR and PPARα.
    Dariusz Ratman et al.
    Nucleic acids research, 44(22), 10539-10553 (2016-09-01)
    Adaptation to fasting involves both Glucocorticoid Receptor (GRα) and Peroxisome Proliferator-Activated Receptor α (PPARα) activation. Given both receptors can physically interact we investigated the possibility of a genome-wide cross-talk between activated GR and PPARα, using ChIP- and RNA-seq in primary
    Role of PTEN in Oxidative Stress and DNA Damage in the Liver of Whole-Body Pten Haplodeficient Mice.
    Ezgi Eyluel Bankoglu et al.
    PloS one, 11(11), e0166956-e0166956 (2016-11-29)
    Type 2 diabetes (T2DM) and obesity are frequently associated with non-alcoholic fatty liver disease (NAFLD) and with an elevated cancer incidence. The molecular mechanisms of carcinogenesis in this context are only partially understood. High blood insulin levels are typical in
    A liver-immune coculture array for predicting systemic drug-induced skin sensitization.
    Lor Huai Chong et al.
    Lab on a chip, 18(21), 3239-3250 (2018-09-27)
    Drug-induced skin sensitization is prevalent worldwide and can trigger life-threatening health conditions, such as Stevens Johnson Syndrome. However, existing in vitro skin models cannot adequately predict the skin sensitization effects of drugs administered into the systemic circulation because dermal inflammation产品细节图片2产品细节图片3

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    图标文献和实验
    该产品被引用文献

    Sigma货号W1878-500ml基础培养液(Williams’Medium E)上海睿安生物13611631389

     

    Sigma货号W1878-6✕500ml基础培养液(Williams’Medium E)上海睿安生物13611631389

    Williams′ 培养基E

    With sodium bicarbonate,without ʟ-glutamine and phenol red,liquid,sterile-filtered,suitable for cell culture

    别名:Williams’ E medium

    说明

    一般描述:Williams培养基E由Williams和Gunn在Williams培养基D的基础上改良而成。培养基专用于支持成人肝脏上皮细胞的长期细胞培养。它是改良型最低必需培养基(MEM)，改变了葡萄糖和氨基酸的含量。

    应用:Williams培养基E已用于培养人肝细胞癌(HCC)细胞和肝细胞。

    重悬:添加0.292g/L L-谷氨酰胺。

    其他说明:酚红会干扰一些细胞在低密度或克隆密度下的生长。在使用干细胞或低密度细胞时，请使用这款William's E。

    引用文献图片1

    同行评审论文(部分文献)

    Metabolic Profiling of Human Long-Term Liver Models and Hepatic Clearance Predictions from In Vitro Data Using Nonlinear Mixed-Effects Modeling.
    Nicole A Kratochwil et al.
    The AAPS journal, 19(2), 534-550 (2017-01-05)
    Early prediction of human clearance is often challenging, in particular for the growing number of low-clearance compounds. Long-term in vitro models have been developed which enable sophisticated hepatic drug disposition studies and improved clearance predictions. Here, the cell line HepG2
    Impact of Environmental Chemicals on the Transcriptome of Primary Human Hepatocytes: Potential for Health Effects.
    Robert D Mitchell et al.
    Journal of biochemical and molecular toxicology, 30(8), 375-395 (2016-04-20)
    New paradigms for human health risk assessment of environmental chemicals emphasize the use of molecular methods and human-derived cell lines. In this study, we examined the effects of the insect repellent DEET (N,N-diethyl-m-toluamide) and the phenylpyrazole insecticide fipronil (fluocyanobenpyrazole) on
    Chromatin recruitment of activated AMPK drives fasting response genes co-controlled by GR and PPARα.
    Dariusz Ratman et al.
    Nucleic acids research, 44(22), 10539-10553 (2016-09-01)
    Adaptation to fasting involves both Glucocorticoid Receptor (GRα) and Peroxisome Proliferator-Activated Receptor α (PPARα) activation. Given both receptors can physically interact we investigated the possibility of a genome-wide cross-talk between activated GR and PPARα, using ChIP- and RNA-seq in primary
    Role of PTEN in Oxidative Stress and DNA Damage in the Liver of Whole-Body Pten Haplodeficient Mice.
    Ezgi Eyluel Bankoglu et al.
    PloS one, 11(11), e0166956-e0166956 (2016-11-29)
    Type 2 diabetes (T2DM) and obesity are frequently associated with non-alcoholic fatty liver disease (NAFLD) and with an elevated cancer incidence. The molecular mechanisms of carcinogenesis in this context are only partially understood. High blood insulin levels are typical in
    A liver-immune coculture array for predicting systemic drug-induced skin sensitization.
    Lor Huai Chong et al.
    Lab on a chip, 18(21), 3239-3250 (2018-09-27)
    Drug-induced skin sensitization is prevalent worldwide and can trigger life-threatening health conditions, such as Stevens Johnson Syndrome. However, existing in vitro skin models cannot adequately predict the skin sensitization effects of drugs administered into the systemic circulation because dermal inflammation引用文献图片2引用文献图片3
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    文献支持
    Sigma货号W1878基础培养液(Williams’Medium E)上海睿安生物13611631389
    ￥742.07