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- 2025年07月21日
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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
Dextran sulfate 5 HS Ph. grade
- 库存：
1000
- 供应商：
北京博蕾德生物科技有限公司
- CAS号：
9011-18-1
- 规格：
10g
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文献和实验mRNA In Situ Hybridization with Nick-Translated Probes
mRNA In Situ Hybridization with Nick-Translated Probes Cells are fixed with freshly made 4% formaldehyde in PBS, pH 7.4 for 15 min at room temperature. All solutions should be made in Molecular Biology grade ultrapure water (no RNase
Basic Fluorescent in situ Hybridization (FISH)
using nick translation random priming or the polymerase chain reaction Hybridization mix solution: 50% formamide [Details：Product No. F7508], 10% dextran sulfate [Details：Product No. D8906], 0.1% SDS [Details：Product No. L4390
96-well RNA In Situ Hybridization Protocol
well plates.III. HYBRIDIZATION IN 96 WELL PLATES 1. Prepare fixed embryos as described in section I. 2. Add 4 ml hybridization buffer WITHOUT dextran sulfate to 1.5ml of settled embryos. 3. Rock embryos for at least 1 hr at RT. 4. Using a multichannel
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