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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
Blue dextran 110
- 库存:
1000
- 供应商:
北京博蕾德生物科技有限公司
- CAS号:
87915-38-6
- 规格:
1 g
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文献和实验?wide scale in a single experiment. The protocols described in this unit are based on separation of protein complexes by blue native polyacrylamide electrophoresis (BN?PAGE), the most versatile native gel system, and the closely related milder
Single tube confirmation PCR protocol
. For example, add 5 µl of the Zymo solution from isolate #1 to PCR tubes 1-5, 5 µl of the Zymo solution from isolate #2 to PCR tubes 6-10 and so on. - Make a PCR master mix by combining: 638 µl water, 110 µl 10 x Taq Buffer, 11 µl NTP's, and 11 µl Taq
In Vivo Measurements of Cytosolic Calcium in Dictyostelium discoideum
:4846–4854, 2000). Measurements of cytosolic Ca2+ ([Ca2+ ]c ) responses have involved the use of different Ca2+ probes including ectopically expressed aequorin (a Ca2+ -sensitive photoprotein), the fluorescent dye fura-2-dextran and the radioisotope 45
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