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文献和实验Semi-synthesis of Glycoproteins from E. coliThrough Native Chemical Ligation
Sufficient quantities of homogeneous samples of post-translationally modified proteins are often not readily available from biological sources to facilitate structure–function investigations. Native chemical ligation (NCL) is a convenient
Expressed Protein Ligation for Protein Semisynthesis and Engineering
Over the past decade, a significant methodological development in peptide ligation strategies has been elaborated that now permits the assembly of peptides and proteins. Native chemical ligation (NCL) has been introduced to join synthetic
Molecular Analysis of p94 and Its Application to Diagnosis of Limb Girdle Muscular Dystrophy Type 2A
p94 (also called calpain 3, nCL-1, or CAPN3) is a calpain large subunit homologue, which is predominantly expressed in skeletal muscle (1 –4 ). The mRNA level of p94 in skeletal muscle is at least 10 times higher
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