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- 2025年07月21日
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文献和实验Detection of Histone H3 Phosphorylation in Cultured Cells and Tissue Sections by Immunostaining
Growth factor stimulation results in phosphorylation of histone H3 at ser 10 and this correlated with expression of immediate early genes suggesting that this phosphorylation is associated with transcriptional activation. Although Western
Monoclonal Antibody Production
. (7) Centrifuge samples at 10,000g 10 min. (8) Pipet off the serum supernatant.Store at -20 ℃.This is your pre-bleed control. Hybridoma Fusion 2. For one fusion you will need (1) 8500 ml bottles of DMEM media (2) 3500 ml bottles of fetal calf serum (FCS
FACS-Based Detection of Phosphorylated Histone H3 for the Quantitation of Mitotic Cells
scanner (FACS) is described, based on the presence of an intranuclear antigen present only in mitotic cells, detected using a specific, commercially available antibody. Cell staining and FACS analysis can be done in a single day, making this a rapid
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