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文献和实验Measuring APP Carboxy-Terminal Fragments
The accumulation of the amyloid-β (Aβ) peptide in the form of insoluble fibrillar deposits and soluble oligomeric aggregates is widely believed to play a causal role in Alzheimer’s disease (AD). Proteolytic cleavage of APP by the β-site APP
羧末端分析 carboxy-terminal analysis
指位于多肽链的羧末端氨基酸残基的分析。化学方法有肼解法( hydrazinolysis)与氚标记法。当用羧肽酶作用时,因为可能从羧末端以某种程度切断组成多肽链的氨基酸,而顺序地游离出氨基酸,所以通过此分析可了解羧末端附近的氨基酸排列顺序。
RNA Trans-Splicing for Genodermatoses
Spliceosome-mediated RNA trans -splicing (SMaRT) is a tool that facilitates the recombination of two distinct pre-mRNA molecules. Its application for gene therapeutic purposes has been hindered by laborious procedures to identify gene
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