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文献和实验negative, while the bile ducts are positive.CAM 5.2- Monoclonal antibody against low M.W. cytokeratin (including CK8 and CK18).- Source: Becton-Dickinson.- Dilution 1:8.- Antigen retrieval method: HIER (pressure cooking) using EDTA.- Staining pattern
Staining protocols for placental explant cultures
-cytokeratin 7 (OV-TL 12/30; Dako; use at 1/50). This identifies the outgrowing cells unequivocally as trophoblast. Anticytokeratin 8/18 can be used for marking cells (e.g CAM5.2, Beckton-Dickenson) but beware that some placental fibroblasts express this marker
Staining protocols for placental explant cultures
over the explant, preventing it from drying out. Antibody should be diluted in 4% BSA/PBS. A good positive control antibody is anti-cytokeratin 7 (OV-TL 12/30; Dako; use at 1/50). This identifies the outgrowing cells unequivocally as trophoblast. Anticytokeratin
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