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- 2025年07月21日
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文献和实验Establishing Relationships Between Closely Related Species Using Total Genomic DNA as a Probe
Questions regarding species identification, differentiation, and relationships often emerge in biological investigations. One may need to examine the extent of genetic relationships between closely related species for the purposes of resource
Preparation of Fluorescent DNA Probe from HUMAN mRNA or Total RNA using Direct Incorporation
I. Preparing fluoresenctly labeled cDNA (probe): To anneal primer, mix 2ug of mRNA or 50-100m g total RNA with 4ug of a regular or anchored oligo-dT primer in a total volume of 15.4 ul:
Prehybridization and probe labeling
1 mL TOTAL 200 mL Prehybridize membranes for at least 6 hours to overnight. Probe labeling Heat water to boiling
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