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文献和实验How to Make Simple Solutions and Dilutions
Example 1: Frozen orange juice concentrate is usually diluted with 4 additional cans of cold water (the dilution solvent) giving a dilution factor of 5, i.e., the orange concentrate represents one unit volume to which you have added 4 more cans (same unit
How to Make Simple Solutions and Dilutions
" dilution) entails combining 1 unit volume of diluent (the material to be diluted) + 4 unit volumes of the solvent medium (hence, 1 + 4 = 5 = dilution factor). Example: Frozen orange juice concentrate is usually diluted in 4 additional cans
. 2. Labeling Culture Cells or Cell Suspensions in Flask 1) Use a standard protocol to grow up cell cultures in vitro . 2) Dilute the labeling reagent 1:100 (from concentrate) with complete tissue culture medium. Filter sterilize
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