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文献和实验Western blotting using ECL reagent
during 2 hours. Approximately 10 ml of diluted antibody showed to be sufficient.4. Transfer the membrane into gel box and repeat step # 2.5. Transfer the membrane back to the small container and incubate with anti-mouse or anti-rabbit IgG horseadish peroxidase
T-cell dependent antigen specific in situ staining (Pe)
if you have them). Let them dry approx 1 hour in a dry atmosphere at RT. Antibodies (anti-Pe) in the sections degrade more rapidly than tissue antigens. Proceed with staining within 24 hours or freeze the slides. Antigen-specific staining: (Pe staining of Pe
数次的停点,检查仪器的重现性和稳定性,必要时也可用旋光标准石英管校正仪器的准确度,读数误差应符合规定。反复测3次,取平均值即为零点或停点。 3.2 取出测定管,倒出溶剂,用供试品溶液少量洗涤测定管数次后装满,在测定管两端盖好玻片及螺帽后,勿使有气泡,将两端透光面用擦镜纸擦干,置样品室内,按下【Config】键,再连续按【Prev】键3次,仪器显示处于“Deflection Test”状态,待读数显示稳定后读取供试品溶液的旋光度,然后按动复测按钮【↓】或【↑】键使检偏镜分别向左或向右偏离,待回复
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