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文献和实验Quantitation of Protein:Folin - phenol Reagent Method (Lowry Method)
up more than 5ug. It is one of the most widely used methods in the quantitation of protein. 【 Apparatus 】 Test tubes and test tube shelf, 100ml flasks, Pipets, 721 type spectroscope 【 Reagents 】 1.Folin- phenol reagent
Dickinson) Silica‐coated thin‐layer chromatography (TLC) plates with fluorescent indicator Kieselgel 60F 254 (Dynamic Adsorbents and Sorbent Technologies
)9 Incubate 30 min at 37°C.10 Rinse 3x with 0.05% Tween 80 in 0.01 M PBS, pH 7.4.11 Rinse 3x with HPLC water.12 Add 50 µl of color development reagent(1 tablet of p-nitrophenyl reagent in 5 ml of 10% diethanolamine, pH 9.8) (mix well)13 Incubate 30 min at 37°C
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