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文献和实验Bicinchoninic Acid (BCA) Protein Assay (Smith)
)has suggested that the BCA assay will replace the Lowry because it requires a single step,and the color reagent is stable under alkaline conditions. Both a standard assay for concentrated proteins and a micro assay for dilute protein solutions are described
Quantitation of Protein:Bicinchoninic Acid (BCA) Method
】 1.Apparatus 721-spectrophotometer, Constant temperature water bath tank, Pipettes, Test tubes and test tube shelf 2.Reagent: (1) BCA reagent: ① Reagent A, 1L:Weigh 10g BCA (1%), 20g Na 2 CO 3• H 2 O
tartrate (dihydrate) (1.6 g), made up to 100mL with water, and adjusted to pH 11.25 with 10 M NaOH.?????? 2. Reagent B: BCA (4.0 g) in 100mL of water. 3. Reagent C: CuSO4·5H2O (0.4 g) in 10mL of water. 4. Standard working reagent (SWR): Mix 1 vol
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