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文献和实验Isolation of Microtubules (Bovine Brain)
minutes at 4° C for further clarification. Collect 10 ml of the supernatant and determine the protein content of your sample using the Bradford protein assay (Appendix G ). Use bovine serum albumin or lysozyme for establishing a standard
Isolation of Microtubules (Bovine Brain)
content of your sample using the Bradford protein assay ( Appendix G ). Use bovine serum albumin or lysozyme for establishing a standard curve. Decant the remainder of the crude supernatant into a chilled beaker, and add an equal
Procedures for In Vitro Production of Bovine Embryos
(E2758) BME amino acids solution (B6766) Bovine serum albumin, Fraction V (A3311) Bovine serum albumin, essentially fatty acid free (A6003) Calcium chloride (C7902) Epinephrine (E4250) DL-Lactic acid, sodium salt, 98% syrup (L-
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