16:0-18:1 PE-d31

Proteolytic Labeling With 18O for Comparative Proteomics Studies: Preparation of 18O-Labeled Peptides and the 18O/16O Peptide Mixture

The method reported here uses proteolytic catalysis to introduce two 18 O atoms into the carboxyl termini of peptides in mixtures, and is intended to be part of the work-flow in comparative proteomics strategies. Proteins

Western Blotting with Horseradish Pe

. 3.Centrifuge the homogenate (16,000 x g, 15 C) for 5 minutes to pellet insoluble material, then discard pellet. 4.Dilute an aliquot of the tissue lysate sample at least 10-fold for the BCA (Pierce) protein concentration assay. POLYACRYLAMIDE GEL

PE TurboMass 与 Agilent 5973 对比

PE TurboMass 与 Agilent  5973 对比表      能检测的分子  TurboMass 1-1200 amu(道尔顿),是目前台式质谱中分子量范围最宽的仪器 5973      2-800 amu(道尔顿) 注：质谱能检测的分子量越宽反应出质谱的综合性能越好,可进行分子和生物大大分子测定 线性动态范围 TMS        线性动态范围:105 5973       线性动态范围:104 注：线性动态范围越宽质谱性能越好，适用于复杂体系的定量