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文献和实验Induction of Deoxyribonucleic Acid Damage by Alkylating Agents
centers of DNA and are monofunctional (have one reactive group) or bifunctional (have two reactive groups). Alkylating agents, such as methylmethanesulphonate and N -ethyl-N -nitrosourea, introduce methyl, ethyl, and complicated alkyl groups
ENU Mutagenesis to Generate Genetically Modified Rat Models
relatively scarce. To date, the most efficient approach for generating genetically modified rats has been the target-selected N -ethyl-N -nitrosourea (ENU) mutagenesis-based technology. Here, we describe the detailed protocols for ENU mutagenesis and mutant
Highly Efficient ENU Mutagenesis in Zebrafish
ENU (N -ethyl-N -nitrosourea) mutagenesis is a widely accepted and proven method to introduce random point mutations in the genome. Because there are no targeted knockout strategies available for zebrafish so far, random mutagenesis
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