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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
Mouse IgG1, κ
- 形态:
液体
- 保存条件:
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
- 克隆性:
Monoclonal
- 标记物:
PerCP/Cyanine 5.5
- 适应物种:
Human
- 保质期:
12个月
- 目录编号:
E-AB-F1229D
- 级别:
科研
- 库存:
999
- 供应商:
武汉伊莱瑞特生物科技股份有限公司
- 宿主:
Mouse
- 应用范围:
FCM
- 抗体英文名:
PE Anti-Human CD279/PD-1 Antibody[EH12.2H7]_货号:E-AB-F1229D
- 抗体名:
PE Anti-Human CD279/PD-1 Antibody流式抗体[EH12.2H7]_货号:E-AB-F1229D
- 规格:
100Tests/20Tests/100Tests×2
| 规格: | 100Tests | 产品价格: | ¥2448.0 |
|---|---|---|---|
| 规格: | 20Tests | 产品价格: | ¥1080.0 |
| 规格: | 100Tests×2 | 产品价格: | ¥3744.0 |

PE Anti-Human CD279/PD-1 抗体流式抗体[EH12.2H7]产品名称:PE Anti-Human CD279/PD-1 Antibody[EH12.2H7], 货号:E-AB-F1229D
| 产品描述: | Programmed cell death 1 (PD-1), also known as CD279, is a 55 kD member of the immunoglobulin superfamily. CD279 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) in the cytoplasmic region and plays a key role in peripheral tolerance and autoimmune disease. CD279 is expressed predominantly on activated T cells, B cells, and myeloid cells. PD-L1 and PD-L2 are ligands of CD279 (PD-1) and are members of the B7 gene family. Evidence suggests overlapping functions for these two PD-1 ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. Interaction of CD279 ligands results in inhibition of T cell proliferation and cytokine secretion. |
| Alternate Names | PDCD1;PD1;Protein PD-1;hPD-1; |
| Swissprot | Q15116 |
| Gene ID | 5133 |
| Clone No | EH12.2H7 |
| Host | Mouse |
| Reactivity | Human |
| Application | FCM |
| 同种型 | Mouse IgG1, κ |
| 同种型 Control | PE Mouse IgG1, κ 同种型 Control[MOPC-21] |
| Conjugation | PE |
| Conjugation Information | PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 585/42 nm bandpass filter). |
| Spectrum | |
| 储存方法 缓冲 | Phosphate 缓冲ed solution, pH 7.2, containing 0.09% sodium azide and 1% BSA. |
| 储存方法 | This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze. |
| Expiration date | 12个月 |
| Shipping | 冰袋 |

PE Anti-Human CD279/PD-1 抗体流式抗体[EH12.2H7]labscience®拥有先进的单抗,多抗和流式抗体开发技术平台,提供8500+高品质 抗体, 适用于胞内和胞外蛋白的鉴定、定量和定位研究。抗体已在多种应用中得到验证,包含 流式细胞术(Flow Cytometry)、 免疫印迹(WB)、 免疫组化(IHC/ICC)、 免疫(共)沉淀(IP/Co-IP)、 免疫荧光(IF) 等实验。抗体产品涉及多种生命科学研究领域,包括肿瘤、免疫、心血管、细胞生物学、神经科学、表观遗传学等。Elabscience®始终致力于提高抗体的质量和种类,以保障并加快您的研究进程
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.PE Anti-Human CD279/PD-1 抗体流式抗体[EH12.2H7]产品更多产品信息查看欢迎咨询我们和搜索武汉伊莱瑞特Elabscience官网查看。
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文献和实验T-cell dependent antigen specific in situ staining (Pe)
if you have them). Let them dry approx 1 hour in a dry atmosphere at RT. Antibodies (anti-Pe) in the sections degrade more rapidly than tissue antigens. Proceed with staining within 24 hours or freeze the slides. Antigen-specific staining: (Pe staining of Pe
(hematopoietic stem cells) 和CD279 (PD-1)。 · 将孵育好的样品加入到 1 ml 的 Strep-Tactin®TACS 琼脂糖层析柱上(柱子最大结合能力:1 x 108个细胞)。 · 当样品完全进入柱子后,加入 10 ml Buffer洗脱两次,就可以得到未结合的细胞了。后续使用流式细胞术检测富集的 NK 细胞。 (此处用到的试剂见文末) 结果与讨论 通过使用阴选将非目的细胞去除后,获得了高纯度的目的细胞群。实验用到了 Strep-Tactin®TACS 琼脂糖层析
STM:傅阳心、侯玉柱等发现放疗与免疫治疗的交汇点,兵家必争之地竟是这群细胞
究竟是哪些 T 细胞亚群发挥了上述作用,研究人员使用抗体清除 CD4 + 或 CD8+ T 细胞。结果发现,只有 CD8+ T 细胞的减少能够消除了放疗诱导的 Ter 细胞减少,这表明是 CD8 + 而不是 CD4+ T 细胞发挥作用。 图片来源:Sci. Transl. Med PD-L1 阻断治疗减少肿瘤诱导的 Ter 细胞积累 免疫疗法,包括 PD-L1 和 PD-1 阻断等,是有望治疗许多癌症的方法。PD-L1 阻断能够增强 CD8+ T 细胞的免疫功能,包括干扰素的产生和细胞毒活性的增强
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