Butyl 5-bromo-2-chlorobenzamid

A Historical Overview of Bromo-Substituted DNA and Sister Chromatid Differentiation

The thymidine analogue 5-bromo-2′-deoxyuridine (BrdU) has been widely used to make sister chromatid differentiation (SCD) evident in metaphase chromosomes of cells grown for two cycles in BrdU and, thus, containing varying

Simultaneous Visualization of FISH Signals and Bromo-deoxyuridine Incorporation by Formamide-Free DNA Denaturation

, or other biologically active molecules. In this chapter, we present a technique, which allows identification of specific DNA sequences by fluorescence in situ hybridization (FISH) and simultaneously analyses the incorporation of a thymidine analogue, 5-bromo

疏水相互作用层析

高盐蛋白的分离   基本步骤 疏水作用层析作为一种吸附性层析，主要步骤包括平衡、上样、清洗、洗脱和再生步骤。疏水作用层析一般在高盐环境中上样，经过上样和清洗之后，通过降低洗脱液中的盐浓度逐渐对不同的分子进行洗脱。其流程可参考下图（图13）： 图13 疏水作用层析流程图   填料选择 疏水填料和离子交换填料类似，都是由基架和疏水配基交联而成，常见的疏水填料配基有 Phenyl、Butyl、Octyl 等，常见疏水填料强弱如图所示： 图14 常见疏水填料强弱图   疏水性强弱，在实验初期