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2-8°C
- 保质期：
6个月
- 库存：
100
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- 规格：
1mg
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文献和实验Preparation of Recombinant Protein Spotted Arrays for Proteome‐Wide Identification of Kinase Targets
‐throughput protein expression screening. Genomics 65:1‐8. Cheung, K.H., Hager, J., Nelson, K., White, K., Li, Y., Snyder, M., Williams, K., and Miller, P. 2002. A dynamic approach
DETECTION OF RECOMBINANT PROTEINS BY ELISA
of interest. If the recombinant protein is not soluble in H2 O, dissolve the insoluble pellet in a volume of 7 M urea equal to 1/10 of the bacterial culture volume. Vortex the samples at a medium speed for about 30 minutes (use a vortex setting of 4.5 on the Fisher Vortex Genie
proteins.It is a convenient alternative to sonication for extracting recombinant proteins from the cytoplasm of Escherichia coli cells carrying expression vectors,especially when many proteins are to be processed in parallel. In this article I’ll summarize
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