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- 百欧泰
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- 2025年07月18日
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2-8°C
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文献和实验Selective Isotopic Labeling of Recombinant Proteins Using Amino Acid Auxotroph Strains
as an expression host for selective labeling of proteins. The application of aromatic auxotroph strains of Pichia pastoris to labeling tyrosines in a recombinant protein (galactose oxidase) will be used to illustrate selective-labeling methods.
The Generation of Multicopy Recombinant Strains
The extremely high levels of alcohol oxidase produced from the native AOX1 gene in Pichia pastoris (5–30% of cell protein on induction) suggested that single-copy AOX1 -promoter expression vectors would be sufficient for efficient foreign gene
Preparation of Recombinant Protein Spotted Arrays for Proteome‐Wide Identification of Kinase Targets
Abstract Table of Contents Materials Figures Literature Cited Abstract Protein microarrays
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