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- 技术资料
- 保存条件：
Store at -20℃，6 months.
- 保质期：
Store at -20℃，6 months.
- 英文名：
Dihydromyricetin(10mM in DMSO，Sterile)
- 库存：
999
- 供应商：
北京索莱宝科技有限公司
- CAS号：
27200-12-0
- 规格：
1ml
| CAS | 27200-12-0 |
| 中文名称 | 二氢杨梅素(10mM in DMSO，无菌） |
| 英文名称 | Dihydromyricetin(10mM in DMSO，Sterile) |
| 分子式 | C15H12O8 |
| 分子量 | 320.25 |
| 规格 | 1ml |
| 溶解性 | 请根据自己的实验要求使用。 |
| 外观(性状) | 无菌溶液 |
| 储存条件 | Store at -20℃，6 months. |
| 运输条件 | 冷冻运输 |
| EC | 814-912-9 |
| MDL | MFCD00189451 |
| SMILES | C1=C(C=C(C(=C1O)O)O)[C@@H]2[C@H](C(=O)C3=C(C=C(C=C3O2)O)O)O |
| InChIKey | KJXSIXMJHKAJOD-LSDHHAIUSA-N |
| InChI | InChI=1S/C15H12O8/c16-6-3-7(17)11-10(4-6)23-15(14(22)13(11)21)5-1-8(18)12(20)9(19)2-5/h1-4，14-20，22H/t14-，15+/m0/s1 |
| PubChem CID | 161557 |
| 靶点 | mTOR |
| 通路 | PI3K/Akt/mTOR |
| 背景说明 | 是一种有效的二氢嘧啶酶 (dihydropyrimidinase) 抑制剂。Dihydromyricetin 可通过抑制 mTOR 信号从而激活自噬。Dihydromyricetin 抑制 mTOR 复合体 (mTORC1/2) 形成。 |
| 生物活性 | Dihydromyricetin is a potent inhibitor with an IC50 of 48 μM on dihydropyrimidinase. Dihydromyricetin can activate autophagy through inhibiting mTOR signaling. Dihydromyricetin suppresses the formation of mTOR complexes (mTORC1/2). Dihydromyricetin is also a potent influenza RNA-dependent RNA polymerase inhibitor with an IC50 of 22 μM.[1-4] |
| 细胞实验 | Hippocampus and cortex tissue samples are homogenized in lysis buffer containing 20 mM Tris (pH 7.5)， 135 mM NaCl， 2 mM EDTA， 2 mM DTT， 25 mM β-glycerophosphate， 2 mM sodium pyrophosphate， 10% glycerol， 1% Triton X-100， 1 mM sodium orthovanadate， 10 mM NaF， 10 μg/mL aprotinin， 10 μg/mL leupeptin， and 1 mM PMSF for 30 min on ice and centrifuged at 12000×g at 4°C for 30 min. The supernatant is collected and protein quantification is carried out using a BCA kit. The protein samples are boiled in the presence of sample buffer at 95°C for 5 min. The target protein is separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)， transferred to nitrocellulose membrane， and then probed by corresponding primary and secondary antibodies. Finally， the target protein is visualized by enhanced chemiluminescence (ECL) reagent exposure to X-ray film[2]. |
| 动物实验 | Rats[2]Totally 40 male Sprague-Dawley (SD) rats (age: 8 weeks old; body weight: 160±20 g) are used. The rats are randomly divided into four groups including normal control group， D-gal model group， and D-gal combined with DHM at the doses of 100 and 200 mg/kg-d groups with 10 rats in each group. All rats are housed at the environment with room temperature of 22±2°C and a dark-light cycle (12 h: 12h)， and provided the accessibility to food and water ad libitum. After adapting to new environment for 1 week， the rats from DHM groups are administered with DHM dissolved in distilled water at the designated dosages by gavage once a day at 8:00am for 6 consecutive weeks. The rats from the normal control group are administrated with distilled water. Except from the normal control group， the rats from other groups are subjected to subcutaneous injection of D-gal at the dose of 150 mg/kg.d for 6 consecutive weeks. Each administration of DHM should be 2 h ahead of D-gal injection. |
| 激酶实验 | A rapid spectrophotometric assay is used to determine the enzymatic activity for hydantoinase， allantoinase， dihydroorotase， and imidase. Dihydrouracil， 5-propyl-hydantoin， and phthalimide are used as substrates. Unless explicitly stated otherwise， Dihydrouracil (2 mM) is used as the substrate in the standard assay of dihydropyrimidinase. Briefly， the decrease in absorbancy at 230， 248， and 298 nm is measured upon hydrolysis of Dihydrouracil， 5-propyl-hydantoin， and Phthalimide as the substrate at 25°C， respectively. To start the reaction， the purified dihydropyrimidinase (10-70 μg) is added to a 2 mL solution containing the substrate and 100 mM Tris-HCl (pH 8.0). Substrate hydrolysis is monitored with a UV/vis spectrophotometer. The extinction coefficient of each substrate is determined experimentally by direct measurement with a spectrophotometer. The extinction coefficients of Dihydrouracil， 5-propyl-hydantoin， and Phthalimide are 0.683 mM-1cm-1 at 230 nm， 0.0538 mM-1cm-1 at 248 nm， and 3.12 mM-1cm-1 at 298 nm， respectively. The initial rates of change are a function of enzyme concentration within the absorbance range of 0.01-0.18 min-1. A unit of activity is defined as the amount of enzyme catalyzing the hydrolysis of 1 μmol substrate/min， and the specific activity is expressed in terms of units of activity per milligram of enzyme. The kinetic parameters Km and Vmax are determined from a non-linear plot by fitting the hydrolyzing rate from individual experiments to the Michaelis-Menten equation[1]. |
| 数据来源文献 | [1]. Huang CY. Inhibition of a Putative Dihydropyrimidinase from Pseudomonas aeruginosa PAO1 by Flavonoids and Substrates of Cyclic Amidohydrolases. PLoS One. 2015 May 19;10(5):e0127634. [2]. Chang H， et al. Ampelopsin suppresses breast carcinogenesis by inhibiting the mTOR signalling pathway. Carcinogenesis. 2014 Aug;35(8):1847-54. [3]. Kou X， et al. Ampelopsin attenuates brain aging of D-gal-induced rats through miR-34a-mediated SIRT1/mTORsignal pathway. Oncotarget. 2016 Nov 15;7(46):74484-74495. [4]. Václav Zima， et al. Unraveling the Anti-Influenza Effect of Flavonoids: Experimental Validation of Luteolin and its Congeners as Potent Influenza Endonuclease Inhibitors. Eur J Med Chem. 22 August 2020， 112754. |
| 单位 | 瓶 |
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二氢杨梅素(10mM in DMSO，无菌)
¥430
