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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Stroe at -20℃，6 months.
- 保质期：
Stroe at -20℃，6 months.
- 英文名：
Ginsenoside Rh2(10mM in DMSO,Sterile）
- 库存：
现询
- 供应商：
北京索莱宝科技有限公司
- CAS号：
78214-33-2
- 规格：
1ml
| 基本信息 | |
| CAS | No.78214-33-2 |
| 中文名称 | 人参皂苷Rh2(10mM in DMSO,无菌） |
| 英文名称 | Ginsenoside Rh2(10mM in DMSO,Sterile） |
| 分子式 | C36H62O8 |
| 分子量 | 622.87 |
| 溶解性 | 请根据自己的实验要求使用。 |
| 外观(性状) | 无菌溶液 |
| 储存条件 | Stroe at -20℃，6 months. |
| 靶点 | Caspase |
| 通路 | Apoptosis |
| 背景说明 | Ginsenoside Rh2 诱导 caspase-8 和 caspase-9 活化。Ginsenoside Rh2 以多途径方式诱导癌细胞凋亡。 |
| 生物活性 | Ginsenoside Rh2 induces the activation of caspase-8 and caspase-9. Ginsenoside Rh2 induces cancer cell apoptosis in a multi-path manner.[1-2] |
| In Vitro | Ginsenoside Rh2 induces the activation of two initiator caspases， caspase-8 and caspase-9 in human cancer cells. Ginsenoside Rh2 induces cancer cell apoptosis in a multi-path manner and is therefore a promising candidate for anti-tumor drug development. Ginsenoside Rh2 triggers p53-dependent Fas expression and consequent activation of caspase-8 and p53-independent caspase-9-mediated intrinsic pathway to cause cancer cell death.The cytotoxic activity of Ginsenoside Rh2 in the human tumor cell lines HeLa， SK-HEP-1， SW480， and PC-3 is assessed by MTT. The cell viability of HeLa cells is remarkably inhibited by Ginsenoside Rh2， with an IC50 value of 2.52 μg/mL， whereas SK-HEP-1 and SW480 cells are less sensitive to Ginsenoside Rh2， with IC50 values of 3.15 μg/mL and 4.06 μg/mL， respectively. PC-3 cells are the least vulnerable to Ginsenoside Rh2， with an IC50 value of 7.85 μg/mL， 3-fold higher than HeLa cells[1] |
| 细胞实验 | A total of 15 days following B16-F10 cell injection， tumor sizes from the 3 tumor bearing groups are measured. The tumor sizes in the G-L group and G-H group (G-L and G-H refer to a low or high dose of ginsenoside Rh2 injection) are reduced compared with the tumor group (P[2]. |
| 细胞实验 | Determination of cell viability is performed by using MTT assay， which is used to calculate the growth inhibition induced by increasing concentrations of drug. Briefly， exponentially growing HeLa， SK-HEP-1， SW480， and PC-3 cells are seeded into a 96-well plate at 1×104 cells/well in triplicate. After incubation for 24 h， cells are treated with increasing concentration of Ginsenoside Rh2 (1， 2.5， 5， 7.5 and 10 μg/mL) in serum free media for 48 h. At the end of treatment， 20 μL of MTT (5 mg/mL) is added to each well and incubated for an additional 4 h. The formazan grains formed by viable cells are solubilized with DMSO， and the color intensity is measured at 550 nm with an ELISA reader[1]. |
| 动物实验 | Mice[2] Male C57BL6 mice (3-4 weeks old) are randomly arranged into 4 groups of 80 mice: Tumor group， G-L group， G-H group and Control group. G-L and G-H refer to a low or high dose of ginsenoside Rh2 injection. For the tumor group， G-L group and G-H group， the B16-F10 cell line is injected into the mice. These 3 groups become tumor bearing groups. For the control group， the same volume of PBS is injected instead. Ginsenoside Rh2 is injected into the left back of mice in the G-L and G-H groups. The dose for the G-H group is 0.5 mg/kg or 0.2 mg/kg for G-L group， every 2 days after day 5. PBS is injected in the tumor and control groups at the same time points. |
| 激酶实验 | HeLa， SK-HEP-1， SW480， and PC-3 cells are treated with Ginsenoside Rh2 (7.5 μg/mL) in serum free media for indicated time periods and then are harvested. Fifty micrograms of cell lysates are incubated with 200 nM Ac-DEVD-AFC (for caspase-3)， Ac-IETD-AFC (for caspase-8)， and Ac-LEHD-AFC (for caspase-9) in a reaction buffer containing 20 mM HEPES， pH 7.4， 100 mM NaCl， 10 mM DTT， 0.1% CHAPS， and 10% sucrose at 37°C for 1 h. The reaction is monitored by fluorescence emission at 535 nm and excitation at 405 nm[1]. |
| 数据来源文献 | [1]. Guo XX， et al. p53-dependent Fas expression is critical for Ginsenoside Rh2 triggered caspase-8 activation in HeLa cells. Protein Cell. 2014 Mar;5(3):224-34. [2]. Wang M， et al. Ginsenoside Rh2 enhances the antitumor immunological response of a melanoma mice model. Oncol Lett. 2017 Feb;13(2):681-685. |
| 单位 | 瓶 |
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IG02801 人参皂苷Rh2(10mM in DMSO,无菌） 抑制剂/拮抗剂/激动剂 索莱宝
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