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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
详询
- 保质期:
详询
- 英文名:
Cyclopentyl chloroformate
- 库存:
98
- 供应商:
北京孚博
- CAS号:
50715-28-1
- 规格:
1g
CAS:50715-28-1
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文献和实验2-DE Spot Amino Acid Analysis with 9-Fluorenylmethyl Chloroformate
Amino acid analysis is a powerful and sensitive technique for the determination of the amino acid composition of proteins. In the past, the automation of amino acid analysis was based on postcolumn ninhydrin analysis, e.g., the Beckman 6300
), dabsyl chloride (25 ), and N-α-(2,4-dinitro-5-fluorophenyl)-l-alaninamide (26 ), the postcolumn fluorescence derivatization with o -phthalaldehyde (27 –31 ), the precolumn fluores cence derivatization with 9-fluorenylmethyl chloroformate (32 ,33
(e) of this protocol resuspends the extracted AAs in 250 mM sodium borate buffer pH 8.8 in preparation for AA analysis using 9-fluorenylmethyl chloroformate (Fmoc). This buffer may, however, not be suitable if AA analysis is to be done using
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