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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
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- 英文名:
Cesium thiocyanate
- 库存:
98
- 供应商:
北京孚博
- CAS号:
3444-31-3
- 规格:
1g
CAS:3444-31-3
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文献和实验RNA Extraction by the Guanidine Thiocyanate Procedure
This method relies on the strong chaotropic nature of the reagents involved to completely denature any ribonuclease (RNase) present in the sample. After lysis in guanidine thiocyanate buffers there are two possibilities for isolation
Cesium Chloride Purification of T7
, and very gently resuspend the pellet in 3.5 mL of T7 Buffer. Centrifuge the lysate at 5,000 rpm for 10 min, and keep the supernatant. Pour a cesium chloride step gradient: add 0.5 mL of cesium chloride with a density of 1.6 to the bottom of a centrifuge tube
Large Scale Plasmid Preps: Qiagen/Cesium Method
most of it will pellet along with the bacterial cell wall. However the relative purity of the plasmid DNA will drop if the plasmid is large or at a relatively low copy number in the prep. The combination of a kit prep with cesium banding eliminates this problem
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