- 询价
- ITI Biochem
- 美国
- 53751-62-5
- 2025年07月18日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
详询
- 保质期：
详询
- 英文名：
CARBONYL BIS(L-METHIONINE P-NITROPHENYL ESTER))
- 库存：
98
- 供应商：
北京孚博
- CAS号：
53751-62-5
- 规格：
1g
CAS：53751-62-5
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文献和实验Labeling Tubulin with Tetramethylrhodamine Succinimidyl Ester
.Take a 10-20μl aliquot and dilute with 400μl buffer.Read OD at 556 nm and calculate dye concentration based on the molar extinction coefficient of 50,000.D/P should be around 0.75 per tubulin monomer.
LABELING MUSCLE ACTIN WITH CARBOXYFLUORESCEIN SUCCINIMIDYL ESTER
ratio.Dilute 1:40 with the dialysis buffer and read the OD at 495 nm. D/P = {OD495 x 41 / 60,000} / {(mg/ml)/ 43,000} 8.Dilute to 3-5 mg/ml with the dialysis buffer.Calculate total mg of actin.Drop freeze in liquid N2 after dissolving 2 mg sucrose
were labeled with Hoechst 33342 (H1399, H3570, H21492), tetramethylrhodamine ethyl ester (TMRE, T669) and rhodamine 110, bis-L-aspartic acid amide (R22122) for 15 minutes. Apoptotic cells show green cytosolic fluorescence resulting from cleavage
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