- 询价
- ITI Biochem
- 美国
- 94247-33-3
- 2025年07月18日
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- 保存条件：
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- 保质期：
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- 英文名：
Calcium,(2-ethylhexanoato-O)(isononanoato-O)- (9CI)
- 库存：
98
- 供应商：
北京孚博
- CAS号：
94247-33-3
- 规格：
1g
CAS：94247-33-3
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文献和实验【原创】lentivirus calcium phosphate transfection protocl
calcium phosphate transfection protocl： 1. Split 293FT cells about 30% confluence, overnight. 2. Thaw 2*HBS room temperature (not in 37 C). 3. For 10cm dish: 1.5ml tube, 4ug packaging plasmid A, 4ug packaging plasmid B, 4ug expression
Measurement of Intracellular Free Calcium Ion Concentration in Cell Populations Using Fura- 2
. The first to be used was quin-2: its structure was based on the novel calcium chelator 1,2-bis-(O-aminophenoxyl-ethane-N, N,N′,N′-tetraacetic acid (BAPTA) (7 , 8 ), a double aromatic analog of EGTA. The major problem of inducing a hydrophilic polycarboxylate anion
DNA TRANSFECTION OF EUKARYOTIC CELLS USING CALCIUM PHOSPHATE
5. Leave 3-4 hrs in CO2 incubator (Can be left o/n). 6. Glycerol Shock: Aspirate medium Wash by adding ~3ml medium then aspirate Add 2.5ml 15% glycerol in HEBS. Sit 4 mins at room temp. then aspirate Wash with 5ml medium per plate
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