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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
详询
- 保质期:
详询
- 英文名:
ACETAMIDE,N-(1-ACETYL-1H-IMIDAZOL-4-YL)-
- 库存:
98
- 供应商:
北京孚博
- CAS号:
408517-81-7
- 规格:
1g
CAS:408517-81-7
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文献和实验Liposome-Mediated, Fluorescence-Based Studies of Sphingolipid Metabolism in Intact Cells
, with an acidic pH optimum (4 ) and a neutral, magnesiumdependent (5 -7 ) enzyme; β-glucosidase and α- or β-galactosidase (8 –11 ); N -acetyl hexosaminidase (12 –14 ); neuraminidase (15 ); phospholipase A1 (16 ,17 ), lysophospholipase (18 –20
Small scale His-Tag fusion protein purification under denaturative conditions
of the sonicator) 4 Spin 15min max speed 4°C 5 Transfer supernatant into clean tube: crude extract (keep 40µl for PAGE-SDS) see Equilibration of Ni-NTA agarose ) 7 Add the crude extract to the beads and incubate 4°C / 1h (swirl
)-2-hydroxypropanesulfonic acid 225.3 6.9 6.2-7.6 Imidazol - HCl 68.08 6.95 6.2-7.8 Bis-Tris Propane 1,3-bis [tris (hydroxymethyl)methylamino]propane 282.3 6.8* 6.3-9.5 BES N,N-bis (2-hydroxyethyl)-2-aminoethanesulfonic acid 213.2 7.15
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