ACETAMIDE,2-AMINO-N-[2-(2,5-DI

Enzymatically Catalyzed Conjugation of a Biodegradable Polymer to Proteins and Small Molecules Using Microbial Transglutaminase

amines (acyl acceptors), including the amino group of lysine (Lys). To convert HES into a suitable substrate for rMTG, the polymer was derivatized with either N -carbobenzyloxy glutaminyl glycine (Z-QG) or hexamethylenediamine to act as an acyl donor

Components and Supplementation of Various Culture Media

= Dimethylsulfoxide HEPES = [4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid]         Buffer NEAA = Non-essential Amino Acids N = Nucleosides Feeder/STO Medium DMEM 7% FCS 1X GPS M15 (ES Cell Growth Medium) DMEM 15% FCS 1X

单组分溶液配制

.3 7.55 6.8-8.2 -0.140 DIPSO 3-[N,N-bis (2-hydroxyethyl)amino]-2-hydroxyprpanesulfonic acid 243.3 7.6 7.0-8.2   TAPSO 3-[N-tris (hydroxymethyl)methylamino]-2-hydroxypropanesulfonic acid 259.3 7.6 7.0-8.2   HEPPSO N -(2-hydroxyethyl