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        Components and Supplementation of Various Culture Media

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        1132

        Components and Supplementation of Various Culture Media


        Abbreviations: DMEM = Dulbecco's Modified Eagle's Medium
        FCS = Fetal Calf (or Bovine) Serum
        GPS = Glutamine-Penicillin-Streptomycin
        BME = b-Mercaptoethanol
        DMSO = Dimethylsulfoxide
        HEPES = [4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid]
                Buffer
        NEAA = Non-essential Amino Acids
        N = Nucleosides


        Feeder/STO Medium

        DMEM
        7% FCS
        1X GPS


        M15 (ES Cell Growth Medium)

        DMEM
        15% FCS
        1X GPS
        1X BME


        2X Freezing Medium

        This should be freshly prepared for each use.  Wear gloves when
        working with DMSO.

        60% DMEM
        20% FCS
        20% DMSO


        Injection Medium

        DMEM
        5% FCS
        20 mM HEPES pH 7.2-7.4

        Embryo Culture Medium

        DMEM
        20% FCS
        1X GPS
        1X BME
        1X NEAA
        1X N

        From the Laboratory of Dr. Allan Bradley              
        Baylor College of Medicine, Houston, Texas

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