5-[(3-Carboxy-4-nitrophenyl)di
thio]-2-nitrobenzoic acid (5,5-Dithiobis(2-nitrobenzoic acid))- 询价
- ITI Biochem
- 美国
- 69-78-3
- 2025年07月18日
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- 文献和实验
- 技术资料
- 英文名：
5-[(3-Carboxy-4-nitrophenyl)dithio]-2-nitrobenzoic acid (5,5-Dithiobis(2-nitrobenzoic acid))
- 库存：
98
- 供应商：
北京孚博
- CAS号：
69-78-3
- 规格：
1g
CAS：69-78-3
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文献和实验Preparation Of Peptide-KLH Conjugates For Immunization
have a tendency to get lost after synthesis.(1) Make up 5 mM Ellman's reagent (dithio-bis-2-nitrobenzoic acid) in 0.1M NaPi pH 7.2.(2) Weigh out about 1 mg of peptide into a tared tube.(3) Add 0.5 ml reagent. It should go bright yellow.(4) Dilute the mixture 1/50
Preparation Of Peptide-KLH Conjugates For Immunization
A. Checking Peptide Thiol Groups Do this before thiol coupling, either to KLH or to resin. Peptide thiol groups have a tendency to get lost after synthesis. Make up 5 mM Ellman's reagent (dithio-bis-2-nitrobenzoic acid) in 0.1M NaPi pH 7.2.
Chemical Production of Bispecific Antibodies
upon reduction of interheavy chain disulfide bonds of the F(ab′)2 fragments. Upon reduction, the resulting Fabs are then recombined to form a Fab′ � Fab′ BsAb. 5,5′-dithiobis (2-nitrobenzoic acid) (DTNB) acts to regenerate disulfide bonds between the two Fabs
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