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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
5-(2-AMINOETHYL)DITHIO-2-NITROBENZOIC ACID
- 库存:
98
- 供应商:
北京孚博
- CAS号:
71899-86-0
- 规格:
1g
CAS:71899-86-0
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文献和实验Preparation Of Peptide-KLH Conjugates For Immunization
have a tendency to get lost after synthesis.(1) Make up 5 mM Ellman's reagent (dithio-bis-2-nitrobenzoic acid) in 0.1M NaPi pH 7.2.(2) Weigh out about 1 mg of peptide into a tared tube.(3) Add 0.5 ml reagent. It should go bright yellow.(4) Dilute the mixture 1/50
Preparation Of Peptide-KLH Conjugates For Immunization
A. Checking Peptide Thiol Groups Do this before thiol coupling, either to KLH or to resin. Peptide thiol groups have a tendency to get lost after synthesis. Make up 5 mM Ellman's reagent (dithio-bis-2-nitrobenzoic acid) in 0.1M NaPi pH 7.2.
Isolation of Microtubules (Bovine Brain)
( -aminoethyl Ether) N,N,N',N'-tetraacetic acid) 0.5 mM MgCl Adjust pH to 6.4 8 M Glycerol in MT buffer Virtis homogenizer (or equivalent) Refrigerated centrifuge GTP Bradford protein assay Procedure
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