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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C保存
- 供应商:
依诺赞(江苏)生物科技有限公司
- 规格:
1000U
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文献和实验Mag-Bind® Plasmid Mega Magnetic Protocol
. The culture density should reach 3-4 x 109 per mL. It is strongly recommended that an endA negative strain of E.coli be used for routine plasmid isolation. Examples of such strains include DH5α® and JM109® . 2. Harvest the bacterial cells
E.Z.N.A.® Yeast Plasmid Mini Spin Protocol
and then at 280 nm. The DNA concentration is calculated as follows: DNA concentration = Absorbance260 × 50 × (Dilution Factor) :g/ml Although the binding capacity of HiBind® DNA Column is around 35 ug, the yield of the yeast plasmid depends
E.Z.N.A.® Endo-free Plasmid Mini Kit I Spin Protocol
strain of E.coli be used for routine plasmid isolation. Examples of such strains include DH5"® and JM109®. 2. Pellet 1.5-5 ml bacteria in appropriate vessels by centrifugation at 10,000 × g for 1 min at room temperature. 3. Decant or aspirate
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