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- INNOZYME
- EN1018
- 2026年01月11日
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- 供应商：
依诺赞（江苏）生物科技有限公司
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1000U
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文献和实验The Use of Resolvases T4 Endonuclease VII and T7 Endonuclease I in Mutation Detection
, then heteroduplex species will be generated with a base-pair mismatch at that position (see Fig. 1 ). These mismatches can be bound and the DNA cleaved by at least two resolvase enzymes, T4 endonuclease VII (1 ) and T7 endonuclease I (3 ), both bacteriophage
Partial Endonuclease Digestion
, tubes 2 through 4 contain 20 µl, and tube 5 contains 10 µl (see diagram below). Place the tubes on ice. Add restriction endonuclease (3-10 units of enzyme per µg of DNA) to tube 1, mix quickly, and place the tube on ice. Add 10 µl
Partial Endonuclease Digestion
2 through 4 contain 20 µl, and tube 5 contains 10 µl (see diagram below). Place the tubes on ice. Add restriction endonuclease (3-10 units of enzyme per µg of DNA ) to tube 1, mix quickly, and place the tube on ice. Add 10 µl from tube 1 to tube 2, mix quickly
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