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- ￥3148
- Elabscience
- 武汉
- E-AB-F1104R1
- 2026年05月28日
- FCM
- Rat
- Mouse
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- 详细信息
- 文献和实验
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- 保存条件：
该产品可在2-8°C条件下保存36个月。请避免长时间暴露于光线中，并切勿冷冻。
- 克隆性：
Monoclonal
- 标记物：
Elab Bright Violet 510
- 适应物种：
Mouse
- 保质期：
1年
- 级别：
科研
- 库存：
99
- 供应商：
武汉伊莱瑞特生物科技有限公司
- 宿主：
Rat
- 应用范围：
FCM
- 靶点：
CD8a
- 抗体英文名：
Elab Bright Violet 510 Anti-Mouse CD8a Antibody[53-6.7]
- 抗体名：
Elab Bright Violet 510 Anti-Mouse CD8a Antibody[53-6.7]
- 规格：
50 Tests
Elab Bright Violet 510 Anti-Mouse CD8a Antibody[53-6.7]
| Alternate Names |
T8, Lyt2, Ly-2
|
| Cellular Localization |
Membrane
|
| Clone No | |
| Isotype |
Rat IgG2a, κ
|
| Leadtime |
咨询
|
| Background |
CD8, also known as Lyt-2, Ly-2, or T8, consists of disulfide-linked α and β chains that form the α(CD8a)/β(CD8b) heterodimer and α/α homodimer. CD8a is a 34 kD protein that belongs to the immunoglobulin family. The CD8 α/β heterodimer is expressed on the surface of most thymocytes and a subset of mature TCR α/β T cells. CD8 expression on mature T cells is non-overlapping with CD4. The CD8 α/α homodimer is expressed on a subset of γ/δ TCR-bearing T cells, NK cells, intestinal intraepithelial lymphocytes, and lymphoid dendritic cells. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 promotes T cell activation through its association with the TCR complex and protein tyrosine kinase lck.
|
| Abbre |
CD8a
|
| Swissprot |
P01731
|
| Host |
Rat
|
| Reactivity |
Mouse
|
| Clonality |
Monoclonal
|
| Isotype Control | |
| Applications | |
| Form |
Liquid
|
| Concentration |
5 μL/Test
|
| Conjugation |
Elab Bright Violet 510
|
| Conjugation Information |
Elab Bright Violet 510 is designed to be excited by the violet laser (405 nm) and detected using an optical filter centered near 510 nm (e.g., a 525/50 nm bandpass filter).
|
| Spectrum |
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
|
| Expiration Date |
12个月
|
| Shipping |
冰袋
|
| Gene ID |
12525
|
Elab Bright Violet 510 Anti-Mouse CD8a Antibody[53-6.7]Elabscience®上线三种新荧光标记（Elab Bright™ Violet 421、Elab Bright™ Violet 510、Elab Bright™ Violet 650）流式抗体53支。目前，Elabscience®已拥有21种标记的流式抗体。
Elab Bright Violet 510 Anti-Mouse CD8a Antibody[53-6.7]2-8℃保存12个月
Elab Bright Violet 510 Anti-Mouse CD8a Antibody[53-6.7]产品更多产品信息查看欢迎咨询我们和搜索武汉伊莱瑞特Elabscience官网查看。
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肿瘤微环境流式结果总是不稳定？你可能忽略了这几个关键细节（二）
于细胞表面。 结果就是：假阳性信号增加；背景升高；细胞分群不清晰或不准确。在肿瘤微环境中经常分析的单核细胞、巨噬细胞、树突状细胞群中尤为明显。（图1） 封闭步骤： 依据样本种属选择对应封闭抗体，在肿瘤组织解离细胞重悬后、染色抗体 cocktail 添加前完成预封闭： 小鼠荷瘤模型 TME 细胞：100 μL 细胞悬液加入 0.5-1 μg 抗小鼠 CD16/CD32 封闭抗体，2-8℃预孵育 10-20 min； 人实体瘤样本：100 μL 体系添加 20 μL 人 Fc
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