相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 供应商:
齐源
- 库存:
999
- 靶点:
IgD
- 级别:
anti-mouse IgD Antibody
- 目录编号:
anti-mouse IgD Antibody
- 克隆性:
单克隆
- 抗原来源:
大鼠
- 保质期:
anti-mouse IgD Antibody
- 抗体英文名:
Brilliant Violet 510™ anti-mouse IgD Antibody
- 抗体名:
IgD 抗体
- 标记物:
anti-mouse IgD Antibody
- 宿主:
大鼠
- 适应物种:
小鼠
- 免疫原:
anti-mouse IgD Antibody
- 亚型:
anti-mouse IgD Antibody
- 形态:
anti-mouse IgD Antibody
- 应用范围:
FC
- 保存条件:
2-8℃
- 浓度:
0.2 mg/ml
- 规格:
50 ug
Description Surface IgD is an important B cell differentiation marker.
Verified Reactivity :Mouse
Antibody Type :Monoclonal
Host Species :Rat
Formulation :Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation :The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510™under optimal conditions.
Concentration :0.2 mg/ml
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application :FC - Quality tested
Recommended Usage :Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cellsin 100 µl volume. It is recommended that the reagent be titrated for optimal performance for eachapplication.
Brilliant Violet 510™ excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm isrecommended for detection, although filter optimization may be required depending on otherfluorophores used. Be sure to verify that your cytometer configuration and software setupare appropriate for detecting this channel. Refer to your instrument manual or manufacturerfor support. Brilliant Violet 510™ is a trademark of Sirigen Group Ltd.
Excitation Laser :Violet Laser (405 nm)
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验1. Nitschke L, et al. 1993. P. Natl. Acad. Sci. USA 90:1887. (FC)
2. Weih D, et al. 2001. J. Immunol. 167:1909. (IHC)
3. Koni PA, et al. 2001. J. Exp. Med. 193:741. (IHC)
4. Ahuja A, et al. 2007. J. Immunol. 179:3351. (FC) PubMed
5. Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
6. Good-Jacobson KL, et al. 2010. Nat. Immunol. 11:535. (FC) PubMed
7. Tomayko MM, et al. 2010. J. Immunol. 185:7146. PubMed
8. Park SY, et al. 2013. J. Immunol. 190:1094. PubMed
9. Rouaud P, et al. 2014. J Exp Med. 211:975. PubMed
10. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
11. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations:
1. Wong R, et al. 2020. Immunity. 53(5):1078-1094.e7. PubMed
2. Yewdell WT, et al. 2020. Immunity. 53(5):952-970.e11. PubMed
3. Campbell C et al. 2018. Immunity. 48(6):1245-1257 . PubMed
4. Abbott RK, et al. 2018. Immunity. 48:133. PubMed
5. Kräutler NJ, et al. 2020. Cell Reports. 30(4):997-1012.e6.. PubMed
6. Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed
7. Vijay R, et al. 2021. J Exp Med. 218:. PubMed
8. Hong JP, et al. 2020. Cell Reports Medicine. 1(3):100035. PubMed
9. Sabouri Z, et al. 2016. Nat Commun. 7:13381. PubMed
10. Vijay R, et al. 2020. Nat Immunol. 21:790. PubMed
11. Monzon-Casanova E, et al. 2021. Eur J Immunol. 51:2266. PubMed
12. Trindade BC, et al. 2021. Immunity. 54:2273. PubMed
流式抗体的选择和配色原则由于在流式细胞实验过程中,荧光抗体对单细胞悬液的标记效果直接影响实验的数据质量。因此,需要考虑各种影响流式抗体品质及检测效果的因素,例如抗体特异性、荧光素信号强弱、荧光素标记方式、同型对照等。本文介绍如何选择合适的流式抗体: 1、需要满足抗体选择的最基本条件:目标蛋白特异性,反应种属以及应用实验等。 靶标特异性:确定标记物名称,确定是细胞表面表达,还是细胞内表达。细胞内表达需要进行透膜。 物种来源:确定待测样本的种属来源。例如,抗小鼠的抗体,不能用于人的样本的检测
R&D Systems 适用于神经细胞培养的无血清培养基添加剂
。 海马神经元表现出突触标记的可靠表达。e18大鼠海马神经元用添加了N21-MaX Media Supplement(目录号ar008)的培养基在体外培养21天。(a) 利用小鼠抗大鼠突触结合蛋白-1单克隆抗体(目录号MaB4364),以及Northernlights™ 557结合的驴抗小鼠igg二抗(目录号Nl007;黄色)对细胞染色,或(B) 利用小鼠抗人caM激酶iiα单克隆抗体(目录号MaB5584),接着用Northernlights™ 557结合的驴抗小鼠 igg二抗(目录号Nl
-His6 抗体(Qiagen) 。 ( 4 ) 特异单克隆抗体,如大鼠抗 CPI 抗体(Affinity Bioreagents,Cholden, USA ) 。 ( 5 ) Cy3 标记的配体作为第二抗体,如兔抗小鼠 IgG 配抗 RGS-His6 —抗,或兔抗大鼠 IgG 配抗 TCP1 —抗( Dianova,Hamburg,Germany) 。 2. 在 FAST 点样片基上的多克隆抗体筛选 ( 1 ) TBS + Tween-20 ( TBST ) :10










